A comprehensive comparison between camelid nanobodies and single chain variable fragments

被引：115

作者：

Asaadi, Yasaman ^{[1
]}

Jouneghani, Fatemeh Fazlollahi ^{[2
]}

Janani, Sara ^{[2
]}

Rahbarizadeh, Fatemeh ^{[3
,4
]}

机构：

[1] Univ Tehran, Coll Sci, Dept Biotechnol, Tehran, Iran

[2] Shahid Beheshti Univ, Fac Life Sci & Biotechnol, Dept Cell & Mol Biol, Tehran, Iran

[3] Tarbiat Modares Univ, Fac Med Sci, Dept Med Biotechnol, Tehran, Iran

[4] Tarbiat Modares Univ, Res & Dev Ctr Biotechnol, Tehran, Iran

来源：

BIOMARKER RESEARCH | 2021年 / 9卷 / 01期

基金：

英国科研创新办公室;

关键词：

Single-chain variable fragment (scFv); camelid VHH; nanobody; single-domain antibody; CHIMERIC ANTIGEN RECEPTOR; DOMAIN ANTIBODY; MONOCLONAL-ANTIBODIES; IN-VITRO; LENTIVIRAL VECTORS; BREAST-CANCER; VEGFR2-SPECIFIC NANOBODY; AFFINITY MATURATION; CRYSTAL-STRUCTURE; LOOP STRUCTURES;

D O I：

10.1186/s40364-021-00332-6

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

By the emergence of recombinant DNA technology, many antibody fragments have been developed devoid of undesired properties of natural immunoglobulins. Among them, camelid heavy-chain variable domains (VHHs) and single-chain variable fragments (scFvs) are the most favored ones. While scFv is used widely in various applications, camelid antibodies (VHHs) can serve as an alternative because of their superior chemical and physical properties such as higher solubility, stability, smaller size, and lower production cost. Here, these two counterparts are compared in structure and properties to identify which one is more suitable for each of their various therapeutic, diagnosis, and research applications.

引用

页数：20

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