Expression of Regulatory Proteins in Choroid Plexus Changes in Early Stages of Alzheimer Disease

被引:22
作者
Krzyzanowska, Agnieszka
Garcia-Consuegra, Ines
Pascual, Consuelo
Antequera, Desiree
Ferrer, Isidro
Carro, Eva
机构
[1] Res Inst Hosp, Neurosci Grp, Madrid, Spain
[2] Biomed Res Networking Ctr Neurodegenerat Dis CIBE, Barcelona, Spain
[3] Res Inst Hosp, Prote Unit, Madrid, Spain
[4] IDIBELL Hosp Univ Bellvitge, Inst Neuropatol, Barcelona, Spain
[5] Univ Barcelona, Barcelona, Spain
关键词
2D electrophoresis; Alzheimer disease; Choroid plexus; Early stages; Proteomic analysis; Regulation; AMYLOID-BETA; BLOOD-BRAIN; ANNEXIN A5; DIAGNOSIS; CYTOTOXICITY; INHIBITION; HYPOTHESIS; TRANSPORT; BIOMARKER; DEMENTIA;
D O I
10.1097/NEN.0000000000000181
中图分类号
R74 [神经病学与精神病学];
学科分类号
摘要
Recent studies indicate that the choroid plexus has important physiologic and pathologic roles in Alzheimer disease (AD). To obtain additional insight on choroid plexus function, we performed a proteomic analysis of choroid plexus samples from patients with AD stages I to II (n = 16), III to IV (n = 16), and V to VI (n = 11) and 7 age-matched control subjects. We used 2-dimensional differential gel electrophoresis coupled with mass spectrometry to generate a complete picture of changes in choroid plexus protein expression occurring in AD patients. We identified 6 proteins: 14-3-3 beta/alpha, 14-3-3 is an element of, moesin, proteasome activator complex subunit 1, annexin V, and aldehyde dehydrogenase, which were significantly regulated in AD patient samples (p < 0.05, 91.5-fold variation in expression vs control samples). These proteins are implicated in major physiologic functions including mitochondrial dysfunction and apoptosis regulation. These findings contribute additional significance to the emerging importance of molecular and functional changes of choroid plexus function in the pathophysiology of AD.
引用
收藏
页码:359 / 369
页数:11
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