In the present study, serum concentrations of the thyroid profile (T3, T4 and TSH) were estimated in normal cyclic, estrous and anoestrous buffaloes. The heifers, both in estrous and anoestrous, were in the age range from 16 months to 24 months, and the cyclic buffaloes were aged 3-6 years. The method adopted for the estimation was the EIA method. The T-3 values observed were 3.179 +/- 1.660, 2.936 +/- 1.260 and 2.238 +/- 0.912, The T-4 values observed were 5.920 +/- 1.963, 5.093 +/- 1.614 and 5.500 +/- 1.208, and the TSH values were 0.035 +/- 0.059, 0.038 +/- 0.030, and 0.014 +/- 0.025 in estrous, cyclic and anoestrous buffaloes, respectively. The overall thyroid diagnosis in estrous was 47%, in cyclic, 34%, and in anoestrous, 19%. The occurrence of the thyroid profile in controls and anoestrous varied significantly. The thyroid profile was low in anoestrous buffaloes when compared with the controls. The variation ranged about 15-18% between controls and anoestrous buffaloes.