Production of a thermostable uricase by a novel Bacillus thermocatenulatus strain

被引：43

作者：

Lotfy, Walid A. ^{[1
]}

机构：

[1] Univ Alexandria, Fac Sci, Dept Microbiol, Alexandria, Egypt

来源：

BIORESOURCE TECHNOLOGY | 2008年 / 99卷 / 04期

关键词：

Bacillus thermocatenulatus; uricase; cane molasses; corn steep liquor;

D O I：

10.1016/j.biortech.2007.01.048

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

A novel uricase-producing bacterium was identified based on its 16S rRNA sequence as Bacillus thermocatenulatus. The kinetic constants for this uricase, determined with uric acid as the substrate, were a V-max of 0.99 U/ml of enzyme and a K. of 0.25 mM. After heat treatment at 75 degrees C for 45 min, the uricase retained about 100% of its initial activity. The uric acid showed to be an inducer for uricase production. The effects of different factors on the enzyme production were studied. Pretreated cane molasses and corn steep liquor were the most promising carbon and nitrogen sources, respectively. When the strain was cultured at 30 degrees C at pH 7.0 for 30-36 h, the uricase activity peaked at 1.25 U/ml. (c) 2007 Elsevier Ltd. All rights reserved.

引用

页码：699 / 702

页数：4

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