55S® Bioglass stimulates in vitro osteoblast differentiation and creates a favorable template for bone tissue formation

被引:0
作者
Loty, S
Sautier, JM
Loty, C
Tan, MT
Greenspan, D
Forest, N
机构
[1] Univ Paris 07, Inst Biomed Cordeliers, Fac Chirurg Dent, Lab Biol Odontol, FR-75270 Paris 06, France
[2] US Biomat Corp, Alachua, FL 32615 USA
来源
BIOCERAMICS | 2000年 / 192-1卷
关键词
bioactive glasses; bone bonding; in vitro; osteogenesis;
D O I
10.4028/www.scientific.net/KEM.192-195.605
中图分类号
TQ174 [陶瓷工业]; TB3 [工程材料学];
学科分类号
0805 ; 080502 ;
摘要
In this study, wt have investigated the behavior of fetal rat osteoblasts cultured on bioactive glasses with 55wt% silica content (55S) and on a bioinert glass (60S). When rat bone cells were cultured on both substrata, they formed multi-layered nodular structures by day 10 in culture. In addition, the specific activity of AP determined biochemically was significantly higher in 55S cultures than in the controls. SEM observations of the material surfaces after scraping-off the cell layers showed that mineralized bone nodules remained attached on 55S surfaces but not on 60S. The 55S/bone interfaces were also analyzed on transverse sections. The interfacial analysis showed a firm bone bonding to the 55S surface through an intervening apatite layer, confirmed by the X-ray mappings. All these results indicated the importance of the surface composition in supporting differentiation of osteogenic cells and the subsequent apposition of bone matrix allowing a strong bond of the bioactive materials to bone.
引用
收藏
页码:605 / 608
页数:4
相关论文
共 8 条
  • [1] AUGER SPECTROSCOPIC ANALYSIS OF BIOGLASS CORROSION FILMS
    CLARK, AE
    PANTANO, CG
    HENCH, LL
    [J]. JOURNAL OF THE AMERICAN CERAMIC SOCIETY, 1976, 59 (1-2) : 37 - 39
  • [2] DIRECT CHEMICAL BOND OF BIOACTIVE GLASS-CERAMIC MATERIALS TO BONE AND MUSCLE
    HENCH, LL
    PASCHALL, HA
    [J]. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH, 1973, 7 (03): : 25 - 42
  • [3] Loty C, 2000, J BIOMED MATER RES, V49, P423, DOI 10.1002/(SICI)1097-4636(20000315)49:4<423::AID-JBM1>3.0.CO
  • [4] 2-7
  • [5] MINERALIZATION INVITRO OF MATRIX FORMED BY OSTEOBLASTS ISOLATED BY COLLAGENASE DIGESTION
    NEFUSSI, JR
    BOYLEFEVRE, ML
    BOULEKBACHE, H
    FOREST, N
    [J]. DIFFERENTIATION, 1985, 29 (02) : 160 - 168
  • [6] COMPOSITIONAL DEPENDENCE OF THE FORMATION OF CALCIUM-PHOSPHATE FILMS ON BIOGLASS
    OGINO, M
    OHUCHI, F
    HENCH, LL
    [J]. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH, 1980, 14 (01): : 55 - 64
  • [7] HISTOLOGICAL AND BIOCHEMICAL EVALUATION OF OSTEOBLASTS CULTURED ON BIOACTIVE GLASS, HYDROXYLAPATITE, TITANIUM-ALLOY, AND STAINLESS-STEEL
    VROUWENVELDER, WCA
    GROOT, CG
    DEGROOT, K
    [J]. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH, 1993, 27 (04): : 465 - 475
  • [8] BETTER HISTOLOGY AND BIOCHEMISTRY FOR OSTEOBLASTS CULTURED ON TITANIUMDOPED BIOACTIVE GLASS - BIOGLASS 45S5 COMPARED WITH IRON-CONTAINING, TITANIUM-CONTAINING, FLUORINE-CONTAINING AND BORON-CONTAINING BIOACTIVE GLASSES
    VROUWENVELDER, WCA
    GROOT, CG
    DEGROOT, K
    [J]. BIOMATERIALS, 1994, 15 (02) : 97 - 106