PCR-based rapid detection of Mycobacterium tuberculosis in blood from immunocompetent patients with pulmonary tuberculosis

被引：40
作者：
Ahmed, N
Mohanty, AK
Mukhopadhyay, U
Batish, VK
Grover, S
机构：
[1] Ctr DNA Fingerprinting & Diagnost, Hyderabad 500007, Andhra Pradesh, India
[2] Natl Dairy Res Inst, Mol Biol Unit, Karnal 132001, Haryana, India
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 1998年 / 36卷 / 10期
关键词：
D O I：
10.1128/JCM.36.10.3094-3095.1998
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A PCR test based on insertion sequence IS1081 was developed to detect Mycobacterium tuberculosis complex organisms in the peripheral blood. The method was applied to blood samples from immunocompetent individuals Frith localized pulmonary tuberculosis. Seven of 16 (43.75%) blood samples were found to be positive for the circulating DNA copies of M. tuberculosis complex.
引用
收藏
页码：3094 / 3095
页数：2
相关论文
共 14 条
  • [1] AHMED N, 1997, THESIS NATL DAIRY RE
  • [2] BRISSONNOEL A, 1992, LANCET, V340, P1069
  • [3] Rapid diagnosis of Mycobacterium tuberculosis bacteremia by PCR
    Folgueira, L
    Delgado, R
    Palenque, E
    Aguado, JM
    Noriega, AR
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1996, 34 (03) : 512 - 515
  • [4] THE RIBOSOMAL INTERGENIC SPACER REGION - A TARGET FOR THE PCR BASED DIAGNOSIS OF TUBERCULOSIS
    GLENNON, M
    SMITH, T
    CORMICAN, M
    NOONE, D
    BARRY, T
    MAHER, M
    DAWSON, M
    GILMARTIN, JJ
    GANNON, F
    [J]. TUBERCLE AND LUNG DISEASE, 1994, 75 (05): : 353 - 360
  • [5] KHANDEKAR P, 1994, INDIAN J MED RES, V100, P167
  • [6] DETECTION OF MYCOBACTERIUM-TUBERCULOSIS IN PERIPHERAL-BLOOD
    KOLK, AHJ
    KOX, LFF
    KUIJPER, S
    RICHTER, C
    [J]. LANCET, 1994, 344 (8923) : 694 - 694
  • [7] MORE RELIABLE PCR FOR DETECTION OF MYCOBACTERIUM-TUBERCULOSIS IN CLINICAL-SAMPLES
    KOX, LFF
    RHIENTHONG, D
    MIRANDA, AM
    UDOMSANTISUK, N
    ELLIS, K
    VANLEEUWEN, J
    VANHEUSDEN, S
    KUIJPER, S
    KOLK, AHJ
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1994, 32 (03) : 672 - 678
  • [8] QUESTIONABLE RELIABILITY OF THE POLYMERASE CHAIN-REACTION IN THE DETECTION OF MYCOBACTERIUM-TUBERCULOSIS
    NOORDHOEK, GT
    VANEMBDEN, JDA
    KOLK, AHJ
    [J]. NEW ENGLAND JOURNAL OF MEDICINE, 1993, 329 (27) : 2036 - 2036
  • [9] AMPLIFICATION OF MYCOBACTERIUM-TUBERCULOSIS FROM PERIPHERAL-BLOOD
    ROLFS, A
    BEIGE, J
    FINCKH, U
    KOHLER, B
    SCHABERG, T
    LOKIES, J
    LODE, H
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1995, 33 (12) : 3312 - 3314
  • [10] AMPLIFICATION OF DNA OF MYCOBACTERIUM-TUBERCULOSIS FROM PERIPHERAL-BLOOD OF PATIENTS WITH PULMONARY TUBERCULOSIS
    SCHLUGER, NW
    CONDOS, R
    LEWIS, S
    ROM, WN
    [J]. LANCET, 1994, 344 (8917) : 232 - 233
12