Silica-immobilized enzymes for multi-step synthesis in microfluidic devices

被引：61
作者：
Luckarift, Heather R.
Ku, Bosung S.
Dordick, Jonathan S.
Spain, Jim C. [1 ]
机构：
[1] Rensselaer Polytech Inst, Dept Chem & Biol Engn, Troy, NY 12180 USA
[2] USAF, Res Lab, Tyndall AFB, FL 32403 USA
[3] Georgia Inst Technol, Sch Civil & Environm Engn, Atlanta, GA 30332 USA
来源：
BIOTECHNOLOGY AND BIOENGINEERING | 2007年 / 98卷 / 03期
关键词：
microfluidics; immobilized enzyme; amino-phenoxazinone; chips; sequential catalysis;
D O I：
10.1002/bit.21447
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The combinatorial synthesis of 2-aminophenoxazin-3-one (APO) in a microfluidic device is reported. Individual microfluidic chips containing metallic zinc, silica-immobilized hydroxylaminobenzene mutase and silica-immobilized soybean peroxidase are connected in series to create a chemo-enzymatic system for synthesis. Zinc catalyzes the initial reduction of nitrobenzene to hydroxylaminobenzene which undergoes a biocatalytic conversion to 2-aminophenol, followed by enzymatic polymerization to APO. Silica-immobilization of enzymes allows the rapid stabilization and integration of the biocatalyst within a microfluidic device with minimal preparation. The system proved suitable for synthesis of a complex natural product (APO) from a simple substrate (nitrobenzene) under continuous flow conditions.
引用
收藏
页码：701 / 705
页数：5
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