All-reflective multiphoton microscope

被引：14
|
作者：
Amirsolaimani, Babak [1 ]
Cromey, Benjamin [1 ]
Peyghambarian, N. [1 ]
Kieu, Khanh [1 ]
机构：
[1] Univ Arizona, Coll Opt Sci, Tucson, AZ 85721 USA
来源：
OPTICS EXPRESS | 2017年 / 25卷 / 19期
基金：
美国国家科学基金会;
关键词：
FLUORESCENCE MICROSCOPY; GENERATION MICROSCOPY; DISPERSION; OBJECTIVES;
D O I：
10.1364/OE.25.023399
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
We present the design, construction, and characterization of a multiphoton microscope that uses reflective elements for beam shaping and steering. This compact all reflective design removes the adverse effects of dispersion on laser pulse broadening as well as chromatic aberration in the focusing of broadband and multicolored laser sources. The design of this system is discussed in detail, including aberrations analysis via ray-tracing simulation and opto-mechanical design. The resolution of this mirror based all-reflective microscope is characterized using fluorescent microbeads. The performance of the system at multiple wavelengths is investigated along with some potential multiphoton imaging and writing applications. (C) 2017 Optical Society of America
引用
收藏
页码：23399 / 23407
页数：9
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