MMP23B expression and protein levels in blood and urine are associated with bladder cancer

被引：15

作者：

Allione, Alessandra ^{[1
,2
]}

Pardini, Barbara ^{[1
,2
]}

Viberti, Clara ^{[1
,2
]}

Giribaldi, Giuliana ^{[3
]}

Turini, Stefano ^{[3
]}

Di Gaetano, Cornelia ^{[1
,2
]}

Guarrera, Simonetta ^{[1
,2
]}

Cordero, Francesca ^{[4
]}

Oderda, Marco ^{[5
]}

Allasia, Marco ^{[5
]}

Gontero, Paolo ^{[5
]}

Sacerdote, Carlotta ^{[6
,7
]}

Vineis, Paolo ^{[1
,8
]}

Matullo, Giuseppe ^{[1
,2
]}

机构：

[1] Italian Inst Genom Med IIGM, Via Nizza 52, Turin, Italy

[2] Univ Turin, Dept Med Sci, Turin, Italy

[3] Univ Turin, Dept Oncol, Turin, Italy

[4] Univ Turin, Dept Comp Sci, Turin, Italy

[5] Citta Salute & Sci, Dept Urol, Turin, Italy

[6] Citta Salute & Sci Univ Hosp, Unit Canc Epidemiol, Turin, Italy

[7] Ctr Canc Prevent CPO, Turin, Italy

[8] Imperial Coll London, Sch Publ Hlth, MRC HPA Ctr Environm & Hlth, London, England

来源：

CARCINOGENESIS | 2018年 / 39卷 / 10期

关键词：

MATRIX METALLOPROTEINASES; UROTHELIAL CARCINOMA; POTASSIUM CHANNEL; TRAFFICKING; GUIDELINES; PROSTATE; SURVIVAL; DISEASE; HEALTH; GRADE;

D O I：

10.1093/carcin/bgy098

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Urothelial bladder cancer (UBC) represents a public health problem because of its high incidence/relapse rates. At present, there are no suitable biomarkers for early diagnosis or relapse/progression prognosis. To improve diagnostic accuracy and overcome the disadvantages of current diagnostic strategies, the detection of UBC biomarkers in easily accessible biofluids, such as urine, represents a promising approach compared with painful biopsies. We investigated the levels of MMP23 genes (microarray and qPCR) and protein (western blot and enzyme-linked immunosorbent assay) in a set of samples (blood, plasma and urine) from patients with UBC and controls as biomarkers for this cancer. MMP23B and its pseudogene MMP23A resulted downregulated in blood cells from UBC compared with controls (66 cases, 70 controls; adjusted P-value = 0.02 and 0.03, respectively). In contrast, MMP23B protein levels in plasma (53 UBC, 49 controls) and urine (59 UBC, 57 controls) increased in cases, being statistically significant in urine. MMP23B dosage observed in urine samples was related to both tumor risk classification and grading. As the lack of correlation between mRNA and protein levels could be due to a posttranscriptional regulation mediated by microRNAs (miRNAs), we investigated the expression of urinary miRNAs targeting MMP23B. Five miRNAs resulted differentially expressed between cases and controls. We reported the first evidence of MMP23B secretion in plasma and urine, suggesting a role of this poorly characterized metalloproteinase in UBC as a potential non-invasive biomarker for this cancer. Further analyses are needed to elucidate the mechanism of regulation of MMP23B expression by miRNAs.

引用

页码：1254 / 1263

页数：10

共 43 条

[1] MicroRNA molecular profiling from matched tumor and bio-fluids in bladder cancer [J].

Armstrong, David A. ;

Green, Benjamin B. ;

Seigne, John D. ;

Schned, Alan R. ;

Marsit, Carmen J. .

MOLECULAR CANCER, 2015, 14

[2] Clinical model of lifetime cost of treating bladder cancer and associated complications [J].

Avritscher, Elenir B. C. ;

Cooksley, Catherine D. ;

Grossman, H. Barton ;

Sabichi, Anita L. ;

Hamblin, Lois ;

Dinney, Colin P. ;

Elting, Linda S. .

UROLOGY, 2006, 68 (03) :549-553

[3] EAU Guidelines on Non-Muscle-invasive Urothelial Carcinoma of the Bladder: Update 2016 [J].

Babjuk, Marko ;

Boehle, Andreas ;

Burger, Maximilian ;

Capoun, Otakar ;

Cohen, Daniel ;

Comperat, Eva M. ;

Hernandez, Virginia ;

Kaasinen, Eero ;

Palou, Joan ;

Roupret, Morgan ;

van Rhijn, Bas W. G. ;

Shariat, Shahrokh F. ;

Soukup, Viktor ;

Sylvester, Richard J. ;

Zigeuner, Richard .

EUROPEAN UROLOGY, 2017, 71 (03) :447-461

[4]

Betel D, NUCL ACIDS RES, V36, pD149

[5] Epidemiology and Risk Factors of Urothelial Bladder Cancer [J].

Burger, Maximilian ;

Catto, James W. F. ;

Dalbagni, Guido ;

Grossman, H. Barton ;

Herr, Harry ;

Karakiewicz, Pierre ;

Kassouf, Wassim ;

Kiemeney, Lambertus A. ;

La Vecchia, Carlo ;

Shariat, Shahrokh ;

Lotan, Yair .

EUROPEAN UROLOGY, 2013, 63 (02) :234-241

[6] The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments [J].

Bustin, Stephen A. ;

Benes, Vladimir ;

Garson, Jeremy A. ;

Hellemans, Jan ;

Huggett, Jim ;

Kubista, Mikael ;

Mueller, Reinhold ;

Nolan, Tania ;

Pfaffl, Michael W. ;

Shipley, Gregory L. ;

Vandesompele, Jo ;

Wittwer, Carl T. .

CLINICAL CHEMISTRY, 2009, 55 (04) :611-622

[7] Analytical Performance of ELISA Assays in Urine: One More Bottleneck towards Biomarker Validation and Clinical Implementation [J].

Chatziharalambous, Despina ;

Lygirou, Vasiliki ;

Latosinska, Agnieszka ;

Stravodimos, Konstantinos ;

Vlahou, Antonia ;

Jankowski, Vera ;

Zoidakis, Jerome .

PLOS ONE, 2016, 11 (02)

[8] Tumor size, stage and grade alterations of urinary peptidome in RCC [J].

Chinello, Clizia ;

Cazzaniga, Marta ;

De Sio, Gabriele ;

Smith, Andrew James ;

Grasso, Angelica ;

Rocco, Bernardo ;

Signorini, Stefano ;

Grasso, Marco ;

Bosari, Silvano ;

Zoppis, Italo ;

Mauri, Giancarlo ;

Magni, Fulvio .

JOURNAL OF TRANSLATIONAL MEDICINE, 2015, 13

[9]

DAVIES B, 1993, CANCER RES, V53, P5365

[10]

Di Carlo A, 2006, ONCOL REP, V15, P1321

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