Enhanced mitochondrial membrane potential and ATP synthesis by photobiomodulation increases viability of the auditory cell line after gentamicin-induced intrinsic apoptosis

被引：46
作者：
Chang, So-Young [1 ]
Lee, Min Young [1 ,2 ]
Chung, Phil-Sang [1 ,2 ]
Kim, Sehwan [3 ,4 ]
Choi, Bernard [5 ]
Suh, Myung-Whan [6 ]
Rhee, Chung-Ku [2 ]
Jung, Jae Yun [1 ,2 ]
机构：
[1] Dankook Univ, Beckman Laser Inst Korea, 119 Dandae Ro, Cheonan 31116, Chungnam, South Korea
[2] Dankook Univ, Dept Otolaryngol Head & Neck Surg, Coll Med, 119 Dandae Ro, Cheonan 31116, Chungnam, South Korea
[3] Dankook Univ, Dept Biomed Engn, Coll Med, 119 Dandae Ro, Cheonan 31116, Chungnam, South Korea
[4] Dankook Univ, Beckman Laser Inst Korea, Dankook, South Korea
[5] Univ Calif Irvine, Beckman Laser Inst & Med Clin, Irvine, CA 92612 USA
[6] Seoul Natl Univ Hosp, Dept Otorhinolaryngol Head & Neck Surg, 101 Daehak Ro, Seoul 03080, South Korea
来源：
SCIENTIFIC REPORTS | 2019年 / 9卷 / 1期
基金：
新加坡国家研究基金会;
关键词：
LEVEL LASER THERAPY; INDUCED HEARING-LOSS; NM LASER; OXIDATIVE STRESS; CORTICAL-NEURONS; DOSE-RESPONSE; LIGHT THERAPY; OTOTOXICITY; PROLIFERATION; MECHANISMS;
D O I：
10.1038/s41598-019-55711-9
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Photobiomodulation (PBM) has been suggested to have a therapeutic effect on irreversible hearing loss induced by aminoglycosides, including gentamicin (GM). However, its intracellular mechanism(s) in GM-induced ototoxicity remain poorly understood. In the present study, we investigated the effect of PBM in GM-induced ototoxicity in auditory cells. We tried to characterize the downstream process by PBM, and the process that triggered the increased cell viability of auditory cells. As a result, the effects of PBM against GM-induced ototoxicity by increasing ATP levels and mitochondrial membrane potential was confirmed. These results suggest a theory to explain the therapeutic effects and support the use of PBM for aminoglycoside-induced hearing loss.
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页数：11
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共 42 条
  • [1] Low-level laser therapy: a useful technique for enhancing the proliferation of various cultured cells
    AlGhamdi, Khalid M.
    Kumar, Ashok
    Moussa, Noura A.
    [J]. LASERS IN MEDICAL SCIENCE, 2012, 27 (01) : 237 - 249
  • [2] Functional Hair Cell Mechanotransducer Channels Are Required for Aminoglycoside Ototoxicity
    Alharazneh, Abdelrahman
    Luk, Lauren
    Huth, Markus
    Monfared, Ashkan
    Steyger, Peter S.
    Cheng, Alan G.
    Ricci, Anthony J.
    [J]. PLOS ONE, 2011, 6 (07):
  • [3] Rhodamine 123 as a probe of mitochondrial membrane potential:: evaluation of proton flux through F0 during ATP synthesis
    Baracca, A
    Sgarbi, G
    Solaini, G
    Lenaz, G
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 2003, 1606 (1-3): : 137 - 146
  • [4] Low-level laser therapy with 850 nm recovers salivary function via membrane redistribution of aquaporin 5 by reducing intracellular Ca2+ overload and ER stress during hyperglycemia
    Biswas, Raktim
    Ahn, Jin Chul
    Moon, Jeong Hwan
    Kim, Jungbin
    Choi, Young-Hoon
    Park, So Young
    Chung, Phil-Sang
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 2018, 1862 (08): : 1770 - 1780
  • [5] Boulton M., 1986, Lasers in the Life Sciences, V1, P125
  • [6] Prevention of noise- and drug-induced hearing loss with D-methionine
    Campbell, Kathleen C. M.
    Meech, Robert P.
    Klemens, James J.
    Gerberi, Michael T.
    Dyrstad, Sara S. W.
    Larsen, Deb L.
    Mitchell, Diana L.
    El-Aziz, Mohammed
    Verhulst, Steven J.
    Hughes, Larry F.
    [J]. HEARING RESEARCH, 2007, 226 (1-2) : 92 - 103
  • [7] Calcium imaging in gentamicin ototoxicity: Increased intracellular calcium relates to oxidative stress and late apoptosis
    Chang, Jiwon
    Yang, Ji Yun
    Choi, June
    Jung, Hak Hyun
    Im, Gi Jung
    [J]. INTERNATIONAL JOURNAL OF PEDIATRIC OTORHINOLARYNGOLOGY, 2011, 75 (12) : 1616 - 1622
  • [8] Photobiomodulation promotes adenoviral gene transduction in auditory cells
    Chang, So-Young
    Park, Yong-Ho
    Carpena, Nathaniel T.
    Pham, Tiffany T.
    Chung, Phil-Sang
    Jung, Jae Yun
    Lee, Min Young
    [J]. LASERS IN MEDICAL SCIENCE, 2019, 34 (02) : 367 - 375
  • [9] 830 nm laser irradiation induces varicosity formation, reduces mitochondrial membrane potential and blocks fast axonal flow in small and medium diameter rat dorsal root ganglion neurons: implications for the analgesic effects of 830 nm laser
    Chow, Roberta T.
    David, Monique A.
    Armati, Patricia J.
    [J]. JOURNAL OF THE PERIPHERAL NERVOUS SYSTEM, 2007, 12 (01) : 28 - 39
  • [10] NaHS Protects Cochlear Hair Cells from Gentamicin-Induced Ototoxicity by Inhibiting the Mitochondrial Apoptosis Pathway
    Dong, Yaodong
    Liu, Dongliang
    Hu, Yue
    Ma, Xiulan
    [J]. PLOS ONE, 2015, 10 (08):
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