Enhanced mitochondrial membrane potential and ATP synthesis by photobiomodulation increases viability of the auditory cell line after gentamicin-induced intrinsic apoptosis

被引：49

作者：

Chang, So-Young ^{[1
]}

Lee, Min Young ^{[1
,2
]}

Chung, Phil-Sang ^{[1
,2
]}

Kim, Sehwan ^{[3
,4
]}

Choi, Bernard ^{[5
]}

Suh, Myung-Whan ^{[6
]}

Rhee, Chung-Ku ^{[2
]}

Jung, Jae Yun ^{[1
,2
]}

机构：

[1] Dankook Univ, Beckman Laser Inst Korea, 119 Dandae Ro, Cheonan 31116, Chungnam, South Korea

[2] Dankook Univ, Dept Otolaryngol Head & Neck Surg, Coll Med, 119 Dandae Ro, Cheonan 31116, Chungnam, South Korea

[3] Dankook Univ, Dept Biomed Engn, Coll Med, 119 Dandae Ro, Cheonan 31116, Chungnam, South Korea

[4] Dankook Univ, Beckman Laser Inst Korea, Dankook, South Korea

[5] Univ Calif Irvine, Beckman Laser Inst & Med Clin, Irvine, CA 92612 USA

[6] Seoul Natl Univ Hosp, Dept Otorhinolaryngol Head & Neck Surg, 101 Daehak Ro, Seoul 03080, South Korea

来源：

SCIENTIFIC REPORTS | 2019年 / 9卷 / 1期

基金：

新加坡国家研究基金会;

关键词：

LEVEL LASER THERAPY; INDUCED HEARING-LOSS; NM LASER; OXIDATIVE STRESS; CORTICAL-NEURONS; DOSE-RESPONSE; LIGHT THERAPY; OTOTOXICITY; PROLIFERATION; MECHANISMS;

D O I：

10.1038/s41598-019-55711-9

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Photobiomodulation (PBM) has been suggested to have a therapeutic effect on irreversible hearing loss induced by aminoglycosides, including gentamicin (GM). However, its intracellular mechanism(s) in GM-induced ototoxicity remain poorly understood. In the present study, we investigated the effect of PBM in GM-induced ototoxicity in auditory cells. We tried to characterize the downstream process by PBM, and the process that triggered the increased cell viability of auditory cells. As a result, the effects of PBM against GM-induced ototoxicity by increasing ATP levels and mitochondrial membrane potential was confirmed. These results suggest a theory to explain the therapeutic effects and support the use of PBM for aminoglycoside-induced hearing loss.

引用

页数：11

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