Spatial confinement of multi-enzyme for cascade catalysis in cell-inspired all-aqueous multicompartmental microcapsules

被引：50

作者：

Qu, Qingli ^{[1
]}

Zhang, Xiaoli ^{[1
]}

Yang, Anquan ^{[2
]}

Wang, Jing ^{[2
]}

Cheng, Weixia ^{[3
]}

Zhou, Aying ^{[1
]}

Deng, Yankang ^{[1
]}

Xiong, Ranhua ^{[1
]}

Huang, Chaobo ^{[1
]}

机构：

[1] Nanjing Forestry Univ NFU, Coll Chem Engn, Jiangsu Coinnovat Ctr Efficient Proc & Utilizat Fo, Joint Lab Adv Biomed Mat NFU,UGent, Nanjing 210037, Peoples R China

[2] Zhejiang Osmun Grp Co Ltd, Huzhou 313000, Peoples R China

[3] Childrens Hosp Nanjing Med Univ, Nanjing 210008, Peoples R China

来源：

JOURNAL OF COLLOID AND INTERFACE SCIENCE | 2022年 / 626卷

基金：

中国国家自然科学基金;

关键词：

Multicompartmental microcapsules; Microreactor; Enzymatic cascade reaction; Logic gate; SYSTEMS;

D O I：

10.1016/j.jcis.2022.06.128

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Biocatalytic reaction networks in eukaryotic cells is realized by the immobilized and compartmental multi-enzymatic system. Inspired by the spatial localization of natural cells, multiple enzymes were con-fined within the multicompartmental microcapsules, which were created using a gas-shearing method coupled with surface-triggered in situ gelation strategy. Heterogeneous multicompartmental (two-, three-, four-, six-, or eight-faced) core particles, due to their capacity for positional assembly, were encap-suled in alginate hydrogel shells. The generated microcapsules integrate logic network to access complex digital design through a three-step convergent enzymatic cascade reaction as a model, and the capsules with high stability, recyclability and cytocompatibility are ideal enzymatic reactor systems to be used for biomimetic biocatalysis process.(c) 2022 Elsevier Inc. All rights reserved.

引用

页码：768 / 774

页数：7