Real-Time PCR assay and melting curve analysis for identification of diarrheagenic Escherichia coli in paediatric population

被引：0

作者：

Singh, Taru ^{[1
]}

Das, Shukla ^{[1
]}

Ramachandran, V. G. ^{[1
]}

Maroof, Khan Amir ^{[2
,3
]}

Rai, Arvind ^{[4
]}

机构：

[1] UCMS, Dept Microbiol, New Delhi, India

[2] UCMS, Dept Community Med, New Delhi, India

[3] Guru Teg Bahadur Hosp, New Delhi, India

[4] Natl Ctr Dis Control, Div Biotechnol & Mol Biol, New Delhi, India

来源：

INDIAN JOURNAL OF EXPERIMENTAL BIOLOGY | 2017年 / 55卷 / 12期

关键词：

Diarrhoea; E. coli infections; Real-Time PCR; STOOL SPECIMENS; INDIA;

D O I：

暂无

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

In developing countries, including India, diarrhea causes considerable death among infants and children. Early diagnosis plays a vital role in preventing the fatality rate. Conventional methods for early detection of diarrheagenic E. coli (DEC) are complicated and inaccurate. Hence, in this study we proposed a syber green based Real-Time PCR to determine the prevalence of DEC. Detection of diarrheagenic E. coli using Real-Time Syber Green based PCR assay was performed in 120 isolates including SO isolates from healthy children. Virulent genes were detected in 106 (88.3%) samples [39 (97.5%) and 67 (83.7%)] in cases and controls, respectively. EPEC was found (typical, atypical and bfp) in 87 (72.5%) specimens followed by EAEC (eagg) in 76 (63.3%), ETEC (elt and est) in 55 (45.8%) and EHEC (stx and hyla) in 11 (9.2%) specimens. Melting curve analysis showed distinct peaks for all target genes.

引用

页码：876 / 879

页数：4

共 17 条

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