Novel genetically encoded fluorescent probes enable real-time detection of potassium in vitro and in vivo

被引：144

作者：

Bischof, Helmut ^{[1
]}

Rehberg, Markus ^{[2
]}

Stryeck, Sarah ^{[1
]}

Artinger, Katharina ^{[3
]}

Eroglu, Emrah ^{[1
]}

Waldeck-Weiermair, Markus ^{[1
]}

Gottschalk, Benjamin ^{[1
]}

Rost, Rene ^{[1
]}

Deak, Andras T. ^{[3
]}

Niedrist, Tobias ^{[4
]}

Vujic, Nemanja ^{[1
]}

Lindermuth, Hanna ^{[5
]}

Prassl, Ruth ^{[5
]}

Pelzmann, Brigitte ^{[5
]}

Groschner, Klaus ^{[5
]}

Kratky, Dagmar ^{[1
,6
]}

Eller, Kathrin ^{[3
]}

Rosenkranz, Alexander R. ^{[3
]}

Madl, Tobias ^{[1
,6
]}

Plesnila, Nikolaus ^{[2
]}

Graier, Wolfgang F. ^{[1
,6
]}

Malli, Roland ^{[1
,6
]}

机构：

[1] Med Univ Graz, Inst Mol Biol & Biochem, Neue Stiftingtalstr 6-6, A-8010 Graz, Austria

[2] Ludwig Maximilians Univ Munchen, Klinikum Univ Munchen, Inst Stroke & Dementia Res ISD, Feodor Lynen Str 17, D-81377 Munich, Germany

[3] Med Univ Graz, Clin Div Nephrol, Auenbruggerpl 27, A-8036 Graz, Austria

[4] Med Univ Graz, Clin Inst Med & Chem Lab Diagnost, Auenbruggerpl 27, A-8036 Graz, Austria

[5] Med Univ Graz, Inst Biophys, Neue Stiftingtalstr 6-4, A-8010 Graz, Austria

[6] BioTechMed Graz, Graz, Austria

来源：

NATURE COMMUNICATIONS | 2017年 / 8卷

基金：

奥地利科学基金会;

关键词：

ENDOPLASMIC-RETICULUM; ION PUMPS; CHANNELS; CA2+; K+; BINDING; HOMEOSTASIS; NA+; DNA; PHYSIOLOGY;

D O I：

10.1038/s41467-017-01615-z

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Changes in intra-and extracellular potassium ion (K+) concentrations control many important cellular processes and related biological functions. However, our current understanding of the spatiotemporal patterns of physiological and pathological K+ changes is severely limited by the lack of practicable detection methods. We developed K+-sensitive genetically encoded, Forster resonance energy transfer-(FRET) based probes, called GEPIIs, which enable quantitative real-time imaging of K+ dynamics. GEPIIs as purified biosensors are suitable to directly and precisely quantify K+ levels in different body fluids and cell growth media. GEPIIs expressed in cells enable time-lapse and real-time recordings of global and local intracellular K+ signals. Hitherto unknown Ca2+-triggered, organelle-specific K+ changes were detected in pancreatic beta cells. Recombinant GEPIIs also enabled visualization of extracellular K+ fluctuations in vivo with 2-photon microscopy. Therefore, GEPIIs are relevant for diverse K+ assays and open new avenues for live-cell K+ imaging.

引用

页数：12

共 51 条

[1] Mitochondrial Ca2+ Uptake 1 (MICU1) and Mitochondrial Ca2+ Uniporter (MCU) Contribute to Metabolism-Secretion Coupling in Clonal Pancreatic β-Cells [J].