Photoacoustic Tomography: In Vivo Imaging from Organelles to Organs

被引:3451
作者
Wang, Lihong V. [1 ]
Hu, Song [1 ]
机构
[1] Washington Univ, Opt Imaging Lab, Dept Biomed Engn, St Louis, MO 63130 USA
关键词
OPTICAL COHERENCE TOMOGRAPHY; MICROSCOPY; RESOLUTION; RECONSTRUCTION; ULTRASOUND;
D O I
10.1126/science.1216210
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Photoacoustic tomography (PAT) can create multiscale multicontrast images of living biological structures ranging from organelles to organs. This emerging technology overcomes the high degree of scattering of optical photons in biological tissue by making use of the photoacoustic effect. Light absorption by molecules creates a thermally induced pressure jump that launches ultrasonic waves, which are received by acoustic detectors to form images. Different implementations of PAT allow the spatial resolution to be scaled with the desired imaging depth in tissue while a high depth-to-resolution ratio is maintained. As a rule of thumb, the achievable spatial resolution is on the order of 1/200 of the desired imaging depth, which can reach up to 7 centimeters. PAT provides anatomical, functional, metabolic, molecular, and genetic contrasts of vasculature, hemodynamics, oxygen metabolism, biomarkers, and gene expression. We review the state of the art of PAT for both biological and clinical studies and discuss future prospects.
引用
收藏
页码:1458 / 1462
页数:5
相关论文
共 54 条
[1]   Whole-body three-dimensional optoacoustic tomography system for small animals [J].
Brecht, Hans-Peter ;
Su, Richard ;
Fronheiser, Matthew ;
Ermilov, Sergey A. ;
Conjusteau, Andre ;
Oraevsky, Alexander A. .
JOURNAL OF BIOMEDICAL OPTICS, 2009, 14 (06)
[2]   Optimization of optode arrangements for diffuse optical tomography: A singular-value analysis [J].
Culver, JP ;
Ntziachristos, V ;
Holboke, MJ ;
Yodh, AG .
OPTICS LETTERS, 2001, 26 (10) :701-703
[3]   Carbon nanotubes as photoacoustic molecular imaging agents in living mice [J].
De La Zerda, Adam ;
Zavaleta, Cristina ;
Keren, Shay ;
Vaithilingam, Srikant ;
Bodapati, Sunil ;
Liu, Zhuang ;
Levi, Jelena ;
Smith, Bryan R. ;
Ma, Te-Jen ;
Oralkan, Omer ;
Cheng, Zhen ;
Chen, Xiaoyuan ;
Dai, Hongjie ;
Khuri-Yakub, Butrus T. ;
Gambhir, Sanjiv S. .
NATURE NANOTECHNOLOGY, 2008, 3 (09) :557-562
[4]   Sentinel Lymph Nodes in the Rat: Noninvasive Photoacoustic and US Imaging with a Clinical US System [J].
Erpelding, Todd N. ;
Kim, Chulhong ;
Pramanik, Manojit ;
Jankovic, Ladislav ;
Maslov, Konstantin ;
Guo, Zijian ;
Margenthaler, Julie A. ;
Pashley, Michael D. ;
Wang, Lihong V. .
RADIOLOGY, 2010, 256 (01) :102-110
[5]   Photoacoustic doppler effect from flowing small light-absorbing particles [J].
Fang, Hui ;
Maslov, Konstantin ;
Wang, Lihong V. .
PHYSICAL REVIEW LETTERS, 2007, 99 (18)
[6]   In vivo photoacoustic microscopy of human cutaneous microvasculature and a nevus [J].
Favazza, Christopher P. ;
Jassim, Omar ;
Cornelius, Lynn A. ;
Wang, Lihong V. .
JOURNAL OF BIOMEDICAL OPTICS, 2011, 16 (01)
[7]   Determining a function from its mean values over a family of spheres [J].
Finch, D ;
Patch, SK ;
Rakesh .
SIAM JOURNAL ON MATHEMATICAL ANALYSIS, 2004, 35 (05) :1213-1240
[8]   On the speckle-free nature of photoacoustic tomography [J].
Guo, Zijian ;
Li, Li ;
Wang, Lihong V. .
MEDICAL PHYSICS, 2009, 36 (09) :4084-4088
[9]  
Hu S., 2010, FRONT NEUROENERG, V2
[10]   Second-generation optical-resolution photoacoustic microscopy with improved sensitivity and speed [J].
Hu, Song ;
Maslov, Konstantin ;
Wang, Lihong V. .
OPTICS LETTERS, 2011, 36 (07) :1134-1136