We present the experimental reconstruction of the Wigner function of an individual electronic spin qubit associated with a nitrogen-vacancy center in diamond at room temperature. This spherical Wigner function contains the same information as the density matrix for arbitrary spin systems. As an example, we exactly characterize the quantum state of a single qubit undergoing a nearly pure dephasing process by the Wigner function. The fidelities and purities during this process are extracted from the experimentally reconstructed Wigner functions, whose dynamics agree with the theoretical prediction. Published under license by AIP Publishing.