Two-dimensional proteome reference maps for the soybean cyst nematode Heterodera glycines

被引:4
|
作者
Chen, Xi [1 ]
MacDonald, Margaret H. [1 ]
Khan, Farooq [1 ]
Garrett, Wesley M. [2 ]
Matthews, Benjamin F. [1 ]
Natarajan, Savithiry S. [1 ]
机构
[1] ARS, USDA, PSI, Soybean Genom & Improvement Lab, Beltsville, MD 20705 USA
[2] Anim Biosci & Biotechnol Lab, Beltsville, MD USA
关键词
2-DE; Animal proteomics; Heterodera glycines; Plant parasitic nematode; Reference map; GEL-ELECTROPHORESIS; PROTEINS; IDENTIFICATION; MAX;
D O I
10.1002/pmic.201100220
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
2-DE reference maps of Heterodera glycines were constructed. After in-gel digestion with trypsin, 803 spots representing 426 proteins were subsequently identified by LC-MS/MS. Proteins with annotated function were further categorized by Gene Ontology. The results showed that proteins involved in metabolic, developmental and biological regulation processes were the most abundant.
引用
收藏
页码:4742 / 4746
页数:5
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