A method for determination of the plasma levels of modafinil enantiomers, (±)-modafinic acid and modafinil sulphone by direct human plasma injection and bidimensional achiral-chiral chromatography

被引:21
作者
Cass, Quezia B. [1 ]
Galatti, Tulio Ferreira [1 ]
机构
[1] Univ Fed Sao Carlos, Dept Quim, BR-13656905 Sao Carlos, SP, Brazil
关键词
modafinil; enantiomers; polysaccharides stationary phases; plasma samples; direct injections;
D O I
10.1016/j.jpba.2007.03.004
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Coupled-column separation using restricted access media as the first dimension in order to exclude macromolecules and retain micromolecules has been successfully used for a number of biological fluids. This paper describes the first method developed and validated for the analysis in a single run of the enantiomers of modafinil and its two major metabolites. The method was developed using a bidimensional HPLC system by coupling a restricted access medium (RAM) bovine serum albumin (BSA) column (1.0 cm x 0.46 cm i.d.) to an amylose tris[(S)-1-phenylethylcarbamate] chiral column. The method was fully validated and showed good linearity, precision, accuracy, sensitivity and selectivity, allowing it to be used for pharmacokinetic studies. The quality of the performance of both columns was maintained with over 280 plasma injections of 100 mu l. (C) 2007 Elsevier B.V. All rights reserved.
引用
收藏
页码:937 / 944
页数:8
相关论文
共 25 条
[1]   Selective and mild oxidation of sulfides to sulfoxides by oxodiperoxo molybdenum complexes adsorbed onto silica gel [J].
Batigalhia, F ;
Zaldini-Hernandes, M ;
Ferreira, AG ;
Malvestiti, I ;
Cass, QB .
TETRAHEDRON, 2001, 57 (48) :9669-9676
[2]   Effect of modafinil on fatigue, mood, and health-related quality of life in patients with narcolepsy [J].
Becker, PM ;
Schwartz, JRL ;
Feldman, NT ;
Hughes, RJ .
PSYCHOPHARMACOLOGY, 2004, 171 (02) :133-139
[3]   Enantiomeric determination of the plasma levels of omeprazole by direct plasma injection using high-performance liquid chromatography with achiral-chiral column-switching [J].
Cass, QB ;
Lima, VV ;
Oliveira, RV ;
Cassiano, NM ;
Degani, ALG ;
Pedrazzoli, J .
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2003, 798 (02) :275-281
[4]   Enantiomeric resolution of a series of chiral sulfoxides by high-performance liquid chromatography on polysaccharide-based columns with multimodal elution [J].
Cass, QB ;
Batigalhia, F .
JOURNAL OF CHROMATOGRAPHY A, 2003, 987 (1-2) :445-452
[5]   Determination of amoxycillin in human plasma by direct injection and coupled-column high-performance liquid chromatography [J].
Cass, QB ;
Gomes, RF ;
Calafatti, SA ;
Pedrazolli, J .
JOURNAL OF CHROMATOGRAPHY A, 2003, 987 (1-2) :235-241
[6]   Enantiomeric determination of pantoprazole in human plasma by multidimensional high-performance liquid chromatography [J].
Cass, QB ;
Degani, ALG ;
Cassiano, NM ;
Pedrazolli, J .
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2002, 766 (01) :153-160
[7]   An enantioselective assay for (±)-modafinil [J].
Cass, QB ;
Kohn, CK ;
Calafatti, SA ;
Aboul-Enein, HY .
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2001, 26 (01) :123-130
[8]   Development of restricted-access media supports and their application to the direct analysis of biological fluid samples via high-performance liquid chromatography [J].
Cassiano, NM ;
Lima, VV ;
Oliveira, RV ;
de Pietro, AC ;
Cass, QB .
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2006, 384 (7-8) :1462-1469
[9]   Determination of the plasma levels of metyrapone and its enantiomeric metyrapol metabolites by direct plasma injection and multidimensional achiral-chiral chromatography [J].
Cassiano, NM ;
Cass, QB ;
Degani, ALG ;
Wainer, IW .
CHIRALITY, 2002, 14 (09) :731-735
[10]   Chiral analysis of d- and l-Modafinil in human serum:: Application to human pharmacokinetic studies [J].
Donovan, JL ;
Malcolm, RJ ;
Markowitz, JS ;
DeVane, CL .
THERAPEUTIC DRUG MONITORING, 2003, 25 (02) :197-202