Association of IL-8-251T/A,+781C/T and+396T/G genetic polymorphisms and haplotypes with breast cancer risk in a Northern Chinese population

Breast cancer is one of the most common cancers among women worldwide. IL-8 belongs to the superfamily of CXC chemokines, and promotes the growth of tumors by elevating angiogenesis. We aimed to investigate the association of IL-8 -251T/A (rs4073), +781C/T (rs2227306) and +396T/G (rs2227307) polymorphisms and haplotypes with breast cancer risk in a Northern Chinese population. A total of 411 breast cancer patients and 411 control subjects were recruited. Genotyping of IL-8 -251T/A (rs4073), +781C/T (rs2227306) and +396T/G (rs2227307) was carried out in a 384-well plate format on the sequenom MassARRAY platform. Demographic, lifestyle and clinical characteristics were collected from medical records and questionnaire interviews. We observed that the AA genotype of IL-8 -251T/A (rs4073) was associated with an increased risk of breast cancer compared to the TT genotype (OR=2.36, 95% CI=1.29-4.32). No association was observed between IL-8 +781C/T (rs2227306) and +396T/G (rs2227307) and risk of breast cancer. The T(251)C(781)T(396) (OR=0.67, 95% CI=0.55-0.83) haplotype was associated with a reduced risk of breast cancer, while the A(251) C(781) T(396) (OR=1.43, 95% CI=1.05-1.94) and A(251) T(781) T(396) (OR=1.50, 95% CI=1.03-2.20) haplotypes were correlated with an elevated risk. This study suggests that the IL-8 -251T/A (rs4073) polymorphism and haplotypes contribute to the risk of breast cancer.