Inducible IL-2 production by dendritic cells revealed by global gene expression analysis

被引:401
|
作者
Granucci, F [1 ]
Vizzardelli, C [1 ]
Pavelka, N [1 ]
Feau, S [1 ]
Persico, M [1 ]
Virzi, E [1 ]
Rescigno, M [1 ]
Moro, G [1 ]
Ricciardi-Castagnoli, P [1 ]
机构
[1] Univ Milano Bicocca, Dept Biosci & Biotechnol, Milan, Italy
关键词
D O I
10.1038/ni0901-882
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Dendritic cells (DCs) are strong activators of primary T cell responses. Their priming ability is acquired upon encounter with maturation stimuli. To identify the genes that are differentially expressed upon maturation induced by exposure to Gram-negative bacteria, a kinetic study of DC gene expression was done with microarrays representing 11,000 genes and ESTs (expressed sequence tags). Approximately 3000 differentially expressed transcripts were identified. We found that functional interleukin 2 (IL-2) mRNA, which gave rise to IL-2 production, was transiently up. regulated at early time-points after bacterial encounter. In contrast, macrophages did not produce IL-2 upon bacterial stimulation. Thus, IL-2 is an additional key cytokine that confers unique T cell stimulatory capacity to DCs.
引用
收藏
页码:882 / 888
页数:7
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