The histone H4 lysine 20 demethylase DPY-21 regulates the dynamics of condensin DC binding

被引:5
作者
Breimann, Laura [1 ,2 ,3 ]
Morao, Ana Karina [1 ]
Kim, Jun [1 ]
Jimenez, David Sebastian [1 ]
Maryn, Nina [1 ]
Bikkasani, Krishna [1 ]
Carrozza, Michael J. [1 ]
Albritton, Sarah E. [1 ]
Kramer, Maxwell [1 ]
Street, Lena Annika [1 ]
Cerimi, Kustrim [2 ]
Schumann, Vic-Fabienne [2 ]
Bahry, Ella [2 ]
Preibisch, Stephan [2 ,4 ]
Woehler, Andrew [2 ]
Ercan, Sevinc [1 ,2 ]
机构
[1] NYU, Ctr Genom & Syst Biol, Dept Biol, New York, NY 10003 USA
[2] Max Delbruck Ctr Mol Med, Berlin Inst Med Syst Biol, D-10115 Berlin, Germany
[3] Humboldt Univ, Inst Biol, D-10099 Berlin, Germany
[4] Howard Hughes Med Inst, Janelia Res Campus, Ashburn, VA 20147 USA
基金
美国国家卫生研究院;
关键词
Condensin; Transcription; Histone modifications; FRAP; Hi-C; C; elegans; DOSAGE COMPENSATION COMPLEX; CELL-CYCLE; CHROMOSOME ARCHITECTURE; GENERAL-METHOD; LIVE-CELL; CHROMATIN; ORGANIZATION; ASSOCIATION; ROLES; FLUOROPHORES;
D O I
10.1242/jcs.258818
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Condensin is a multi-subunit structural maintenance of chromosomes (SMC) complex that binds to and compacts chromosomes. Here, we addressed the regulation of condensin binding dynamics using Caenorhabditis elegans condensin DC, which represses X chromosomes in hermaphrodites for dosage compensation. We established fluorescence recovery after photobleaching (FRAP) using the SMC4 homolog DPY-27 and showed that a well-characterized ATPase mutation abolishes DPY-27 binding to X chromosomes. Next, we performed FRAP in the background of several chromatin modifier mutants that cause varying degrees of X chromosome derepression. The greatest effect was in a null mutant of the H4K2Ome2 demethylase DPY-21, where the mobile fraction of condensin DC reduced from similar to 30% to 10%. In contrast, a catalytic mutant of dpy-21 did not regulate condensin DC mobility. Hi-C sequencing data from the dpy-21 null mutant showed little change compared to wild-type data, uncoupling Hi-C-measured long-range DNA contacts from transcriptional repression of the X chromosomes. Taken together, our results indicate that DPY-21 has a non-catalytic role in regulating the dynamics of condensin DC binding, which is important for transcription repression.
引用
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页数:16
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