Reversible on-demand cell alignment using reconfigurable microtopography

被引:76
作者
Lam, Mai T. [1 ]
Clem, William C. [1 ]
Takayama, Shuichi [1 ]
机构
[1] Univ Michigan, Dept Biomed Engn, Macromol Sci & Engn Program, Ann Arbor, MI 48109 USA
关键词
surface topography; micropatterning; compression; silicone elastomer; poly(dimethylsiloxane); muscle;
D O I
10.1016/j.biomaterials.2007.12.010
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Traditional cell culture substrates consist of static, flat surfaces although in vivo, cells exist on various dynamic topographies. We report development of a reconfigurable microtopographical system compatible with cell culture that is comprised of reversible wavy microfeatures on poly(dimethylsiloxane). Robust reversibility of the wavy micropattern is induced on the cell culture customized substrate by first plasma oxidizing the substrate to create a thin, brittle film on the surface and then applying and releasing compressive strain, to introduce and remove the microfeatures, respectively. The reversible topography was able to align, unalign, and realign C2C12 myogenic cell line cells repeatedly on the same substrate within 24 h intervals, and did not inhibit cell differentiation. The flexibility and simplicity of the materials and methods presented here provide a broadly applicable capability by which to investigate and compare dynamic cellular processes not yet easily studied using conventional in vitro culture substrates. (c) 2007 Elsevier Ltd. All rights reserved.
引用
收藏
页码:1705 / 1712
页数:8
相关论文
共 42 条
  • [1] Interactive effects of surface topography and pulsatile electrical field stimulation on orientation and elongation of fibroblasts and cardiomyocytes
    Au, Hoi Ting H.
    Cheng, Irene
    Chowdhury, Mohammad F.
    Radisic, Milica
    [J]. BIOMATERIALS, 2007, 28 (29) : 4277 - 4293
  • [2] Spontaneous formation of ordered structures in thin films of metals supported on an elastomeric polymer
    Bowden, N
    Brittain, S
    Evans, AG
    Hutchinson, JW
    Whitesides, GM
    [J]. NATURE, 1998, 393 (6681) : 146 - 149
  • [3] The controlled formation of ordered, sinusoidal structures by plasma oxidation of an elastomeric polymer
    Bowden, N
    Huck, WTS
    Paul, KE
    Whitesides, GM
    [J]. APPLIED PHYSICS LETTERS, 1999, 75 (17) : 2557 - 2559
  • [4] Geometry and physics of wrinkling
    Cerda, E
    Mahadevan, L
    [J]. PHYSICAL REVIEW LETTERS, 2003, 90 (07) : 4
  • [5] Structural change in living tissues
    Cowin, SC
    [J]. MECCANICA, 1999, 34 (05) : 379 - 398
  • [6] Topographical control of cells
    Curtis, A
    Wilkinson, C
    [J]. BIOMATERIALS, 1997, 18 (24) : 1573 - 1583
  • [7] Reactions of cells to topography
    Curtis, ASG
    Wilkinson, CD
    [J]. JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION, 1998, 9 (12) : 1313 - 1329
  • [8] Dynamic changes in traction forces with DC electric field in osteoblast-like cells
    Curtze, S
    Dembo, M
    Miron, M
    Jones, DB
    [J]. JOURNAL OF CELL SCIENCE, 2004, 117 (13) : 2721 - 2729
  • [9] The embryogenesis of congenital vertebral dislocation: Early embryonic buckling?
    Dias, MS
    Li, VT
    Landi, M
    Schwend, R
    Grabb, P
    [J]. PEDIATRIC NEUROSURGERY, 1998, 29 (06) : 281 - 289
  • [10] Switchable surface traps for injectable bead-based chromatography in PDMS microfluidic channels
    Ebara, Mitsuhiro
    Hoffman, John M.
    Hoffman, Allan S.
    Stayton, Patrick S.
    [J]. LAB ON A CHIP, 2006, 6 (07) : 843 - 848