The in vitro evolution of BCG vaccines

被引：86
作者：
Mostowy, S
Tsolaki, AG
Small, PM
Behr, MA
机构：
[1] McGill Univ, Hlth Ctr, Montreal, PQ H3G 1A4, Canada
[2] Stanford Univ, Dept Med, Div Infect Dis & Geog Med, Stanford, CA 94305 USA
来源：
VACCINE | 2003年 / 21卷 / 27-30期
基金：
加拿大健康研究院;
关键词：
BCG; GeneChip (TM); tuberculosis;
D O I：
10.1016/S0264-410X(03)00484-5
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
The bacillus Calmette-Geurin (BCG) family of vaccines currently implemented to prevent tuberculosis (TB) consist of clonal bacterial strains independently shaped by nearly a half-century of evolution. Derived from virulent Mycobacterium bovis, daughter strains of BCG were additionally passaged under the same laboratory conditions that resulted in its original attenuation. Genomic loss of the RDI region has been demonstrated to coincide with attenuation from virulence, while deletions occurring after the loss of RDI are speculated to be responsible for BCG's over-attenuation. To provide a more complete description of their total genomic variation, the genomic content of BCG strains are investigated by Affymetrix GeneChip(TM). Because clinical isolates of M. tuberculosis have previously been characterized via GeneChip(TM) interrogation, analysis permits the comparison of in vivo versus in vitro evolution of M. tuberculosis complex subspecies. The contrast between the two modes of evolution are discussed in its relevance towards TB pathogenicity. (C) 2003 Elsevier Ltd. All rights reserved.
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收藏
页码：4270 / 4274
页数：5
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