Enhanced bioanalyte detection in waveguide confined Raman spectroscopy using wavelength modulation

被引：23

作者：

Ashok, Praveen C. ^{[1
]}

De Luca, Anna Chiara ^{[1
]}

Mazilu, Michael ^{[1
]}

Dholakia, Kishan ^{[1
]}

机构：

[1] Univ St Andrews, Sch Phys & Astron, SUPA, St Andrews KY16 9SS, Fife, Scotland

来源：

JOURNAL OF BIOPHOTONICS | 2011年 / 4卷 / 7-8期

基金：

英国工程与自然科学研究理事会;

关键词：

Raman spectroscopy; microfluidics; bioanalyte detection; fluorescent suppression; FLUORESCENCE SUPPRESSION; EXCITATION; FIBER; SCATTERING; REJECTION; PROBE; CHIP;

D O I：

10.1002/jbio.201000107

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Waveguide confined Raman spectroscopy (WCRS) incorporates a fibre based Raman detection system in a microfluidic platform enabling the spectroscopic detection of analyte. It offers the possibility to develop portable, alignment free devices for bio-analyte sensing with minimal sample preparation. Ultimate sensitivity is limited by the fibre auto-fluorescence background. Here we report enhanced bio-analyte detection sensitivity by combining WCRS with continuous wavelength modulation technique. We used urea as a model analyte and the modulation parameters have been optimized to maximize the sensitivity of the device. Schematic diagram of the implementation of modulation scheme in WCRS for fluorescent suppression.

引用

页码：514 / 518

页数：5

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