Identification and analysis of seven effector protein families with different adaptive and evolutionary histories in plant-associated members of the Xanthomonadaceae

被引:30
作者
Assis, Renata de A. B. [1 ]
Polloni, Lorraine Cristina [2 ]
Patane, Jose S. L. [3 ]
Thakur, Shalabh [4 ]
Felestrino, Erica B. [1 ]
Diaz-Caballero, Julio [4 ]
Digiampietri, Luciano Antonio
Goulart, Luiz Ricardo [2 ]
Almeida, Nalvo F. [5 ,6 ]
Nascimento, Rafael [2 ]
Dandekar, Abhaya M. [7 ]
Zaini, Paulo A. [2 ,7 ]
Setubal, Joao C. [3 ]
Guttman, David S. [4 ,8 ]
Moreira, Leandro Marcio [1 ,9 ]
机构
[1] Univ Fed Ouro Preto, Cent Res Biol Sci, Ouro Preto, MG, Brazil
[2] Univ Fed Uberlandia, Inst Genet & Biochem, Uberlandia, MG, Brazil
[3] Univ Sao Paulo, Inst Quim, Dept Bioquim, Sao Paulo, SP, Brazil
[4] Univ Toronto, Dept Cell & Syst Biol, 25 Willcocks St, Toronto, ON M5S 3B2, Canada
[5] Univ Sao Paulo, Escola Artes Ciencias & Humanidades, Sao Paulo, SP, Brazil
[6] Univ Fed Mato Grosso do Sul, Sch Comp, Mato Grosso, MS, Brazil
[7] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA
[8] Univ Toronto, Ctr Anal Genome Evolut & Funct, 25 Willcocks St, Toronto, ON M5S 3B2, Canada
[9] Univ Fed Ouro Preto, Inst Exact & Biol Sci, Dept Biol Sci, Ouro Preto, MG, Brazil
来源
SCIENTIFIC REPORTS | 2017年 / 7卷
关键词
ORYZAE PV. ORYZAE; COMPARATIVE GENOMIC ANALYSIS; MUTASE-PREPHENATE DEHYDRATASE; MULTIPLE SEQUENCE ALIGNMENT; FREE N-GLYCANS; CHORISMATE MUTASE; XYLELLA-FASTIDIOSA; SECRETION SYSTEMS; SIGNAL PEPTIDES; VIRULENCE;
D O I
10.1038/s41598-017-16325-1
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The Xanthomonadaceae family consists of species of non-pathogenic and pathogenic gamma-proteobacteria that infect different hosts, including humans and plants. In this study, we performed a comparative analysis using 69 fully sequenced genomes belonging to this family, with a focus on identifying proteins enriched in phytopathogens that could explain the lifestyle and the ability to infect plants. Using a computational approach, we identified seven phytopathogen-enriched protein families putatively secreted by type II secretory system: PheA (CM-sec), LipA/LesA, VirK, and four families involved in N-glycan degradation, NixE, NixF, NixL, and FucA1. In silico and phylogenetic analyses of these protein families revealed they all have orthologs in other phytopathogenic or symbiotic bacteria, and are involved in the modulation and evasion of the immune system. As a proof of concept, we performed a biochemical characterization of LipA from Xac306 and verified that the mutant strain lost most of its lipase and esterase activities and displayed reduced virulence in citrus. Since this study includes closely related organisms with distinct lifestyles and highlights proteins directly related to adaptation inside plant tissues, novel approaches might use these proteins as biotechnological targets for disease control, and contribute to our understanding of the coevolution of plant-associated bacteria.
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页数:17
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