Molecular mechanism of substrate recognition and transport by the AtSWEET13 sugar transporter

被引：78

作者：

Han, Lei ^{[1
,2
]}

Zhu, Yongping ^{[1
,2
]}

Liu, Min ^{[1
,2
]}

Zhou, Ye ^{[1
,2
]}

Lu, Guangyuan ^{[1
,2
]}

Lan, Lan ^{[1
,2
]}

Wang, Xianping ^{[1
]}

Zhao, Yongfang ^{[1
]}

Zhang, Xuejun C. ^{[1
,2
]}

机构：

[1] Chinese Acad Sci, Inst Biophys, CAS Ctr Excellence Biomacromol, Natl Lab Biomacromol, Beijing 100101, Peoples R China

[2] Univ Chinese Acad Sci, Beijing 100049, Peoples R China

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2017年 / 114卷 / 38期

基金：

中国国家自然科学基金;

关键词：

SWEETs; sugar transporter; uniporter; smFRET; CRYSTAL-STRUCTURE; STRUCTURE REFINEMENT; SWEET TRANSPORTERS; MEMBRANE-PROTEINS; BACTERIAL; TRANSLOCATION; NUTRITION; SEMISWEET; HOMOLOG;

D O I：

10.1073/pnas.1709241114

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Sugar Will Eventually be Exported Transporters (SWEETs) are recently identified sugar transporters that can discriminate and transport di- or monosaccharides across a membrane following the concentration gradient. SWEETs play key roles in plant biological processes, such as pollen nutrition, nectar secretion, seed filling, and phloem loading. SWEET13 from Arabidopsis thaliana (AtSWEET13) is an important sucrose transporter in pollen development. Here, we report the 2.8-angstrom resolution crystal structure of AtSWEET13 in the inward-facing conformation with a substrate analog, 2'-deoxycytidine 5'-monophosphate, bound in the central cavity. In addition, based on the results of an in-cell transport activity assay and single-molecule Forster resonance energy transfer analysis, we suggest a mechanism for substrate selectivity based on the size of the substrate-binding pocket. Furthermore, AtSWEET13 appears to form a higher order structure presumably related to its function.

引用

页码：10089 / 10094

页数：6

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