Wide field-of-view, multi-region, two-photon imaging of neuronal activity in the mammalian brain

被引：241
作者：
Stirman, Jeffrey N. [1 ,2 ]
Smith, Ikuko T. [1 ,3 ]
Kudenov, Michael W. [4 ]
Smith, Spencer L. [1 ,2 ,5 ,6 ]
机构：
[1] Univ N Carolina, Sch Med, Neurosci Ctr, Chapel Hill, NC 27599 USA
[2] Univ N Carolina, Sch Med, Carolina Inst Dev Disabil, Chapel Hill, NC 27599 USA
[3] Univ N Carolina, Sch Med, Dept Pharmacol, Chapel Hill, NC USA
[4] North Carolina State Univ, Dept Elect & Comp Engn, Raleigh, NC USA
[5] Univ N Carolina, Sch Med, Dept Cell Biol & Physiol, Chapel Hill, NC 27599 USA
[6] Univ N Carolina, Sch Med, Neurobiol Curriculum, Chapel Hill, NC 27599 USA
来源：
NATURE BIOTECHNOLOGY | 2016年 / 34卷 / 08期
基金：
美国国家科学基金会;
关键词：
MOUSE VISUAL-CORTEX; CELLULAR RESOLUTION; IN-VIVO; MICROSCOPY; MICE; SPECIFICITY; INFERENCE;
D O I：
10.1038/nbt.3594
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Two-photon calcium imaging provides an optical readout of neuronal activity in populations of neurons with subcellular resolution. However, conventional two-photon imaging systems are limited in their field of view to similar to 1 mm(2), precluding the visualization of multiple cortical areas simultaneously. Here, we demonstrate a two-photon microscope with an expanded field of view (>9.5 mm(2)) for rapidly reconfigurable simultaneous scanning of widely separated populations of neurons. We custom designed and assembled an optimized scan engine, objective, and two independently positionable, temporally multiplexed excitation pathways. We used this new microscope to measure activity correlations between two cortical visual areas in mice during visual processing.
引用
收藏
页码：857 / +
页数：10
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