Magnesium ion is an effective inhibitor of the binding of Escherichia coli to heparin

被引：1
作者：
Kang, Jonghoon [1 ]
Lee, Myung Soog [1 ]
Gorenstein, David G. [1 ]
机构：
[1] Univ Texas, Med Branch, Dedpt Biochem & Mol Biol, Sealy Ctr Struct Biol, Galveston, TX 77555 USA
来源：
JOURNAL OF MICROBIOLOGICAL METHODS | 2007年 / 71卷 / 03期
关键词：
chemiluminescence; EMSA; heparin; heparin-bacteria interaction; host-pathogen interaction; magnesium;
D O I：
10.1016/j.mimet.2007.10.002
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We investigated the effects of ions and temperature on the binding of E. coli to heparin using a chemiluminescence electrophoretic mobility shift assay. We found that magnesium ion is an effective inhibitor of the binding. The method can be readily applied to discover agents that can block the binding. (c) 2007 Elsevier B.V All rights reserved.
引用
收藏
页码：340 / 342
页数：3
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