Molecularly imprinted solid-phase extraction for the selective determination of valnemulin in feeds with high performance liquid chromatography

被引：15

作者：

Guo, Hongbin ^{[1
]}

Liu, Kaiyong ^{[2
]}

Liu, Yahong ^{[1
]}

Fang, Binghu ^{[1
]}

Liu, Min ^{[1
]}

He, Limin ^{[1
]}

Zeng, Zhenling ^{[1
]}

机构：

[1] S China Agr Univ, Coll Vet Med, Dept Pharmacol & Toxicol, Guangzhou 510642, Guangdong, Peoples R China

[2] Henan Univ, Food & Bioengn Dept, Luoyang 471003, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2011年 / 879卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Valnemulin; Feeds; High performance liquid chromatography; Molecularly imprinted polymer; Solid-phase extraction; POLYMERS; SAMPLES;

D O I：

10.1016/j.jchromb.2010.11.041

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple, sensitive and reproducible high performance liquid chromatographic method was developed for determining valnemulin in feeds. Feed samples were extracted with ethyl acetate under alkaline condition, cleaned up by molecularly imprinted solid-phase extraction, and analyzed by high performance liquid chromatography with ultraviolet detection. The characteristics of the synthesized polymer were evaluated and the loading capacity of the polymer was about 1000 mu g analyte/g imprinted polymer. The new procedure for the feed sample cleanup using the prepared polymer cartridge gave higher recoveries and fewer matrix interferences. The assay exhibited a linear dynamic range of 5.0-200 mg kg(-1) with the correlation coefficient above 0.9993. Recoveries of valnemulin from feed samples spiked at 5.0, 20 and 50 mg kg(-1) ranged between 76.0% and 94.4% with relative standard deviations of less than 9%. The limit of detection for valnemulin in feeds was 1 mg kg(-1). (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：181 / 185

页数：5

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