Molecularly imprinted solid-phase extraction for the selective determination of valnemulin in feeds with high performance liquid chromatography

被引：15

作者：

Guo, Hongbin ^{[1
]}

Liu, Kaiyong ^{[2
]}

Liu, Yahong ^{[1
]}

Fang, Binghu ^{[1
]}

Liu, Min ^{[1
]}

He, Limin ^{[1
]}

Zeng, Zhenling ^{[1
]}

机构：

[1] S China Agr Univ, Coll Vet Med, Dept Pharmacol & Toxicol, Guangzhou 510642, Guangdong, Peoples R China

[2] Henan Univ, Food & Bioengn Dept, Luoyang 471003, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2011年 / 879卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Valnemulin; Feeds; High performance liquid chromatography; Molecularly imprinted polymer; Solid-phase extraction; POLYMERS; SAMPLES;

D O I：

10.1016/j.jchromb.2010.11.041

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple, sensitive and reproducible high performance liquid chromatographic method was developed for determining valnemulin in feeds. Feed samples were extracted with ethyl acetate under alkaline condition, cleaned up by molecularly imprinted solid-phase extraction, and analyzed by high performance liquid chromatography with ultraviolet detection. The characteristics of the synthesized polymer were evaluated and the loading capacity of the polymer was about 1000 mu g analyte/g imprinted polymer. The new procedure for the feed sample cleanup using the prepared polymer cartridge gave higher recoveries and fewer matrix interferences. The assay exhibited a linear dynamic range of 5.0-200 mg kg(-1) with the correlation coefficient above 0.9993. Recoveries of valnemulin from feed samples spiked at 5.0, 20 and 50 mg kg(-1) ranged between 76.0% and 94.4% with relative standard deviations of less than 9%. The limit of detection for valnemulin in feeds was 1 mg kg(-1). (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：181 / 185

页数：5

共 24 条

[1] The use of molecularly imprinted polymers for extraction of sulfonylurea herbicides [J].

Bastide, J ;

Cambon, JP ;

Breton, F ;

Piletsky, SA ;

Rouillon, R .

ANALYTICA CHIMICA ACTA, 2005, 542 (01) :97-103

[2] Extraction of clenbuterol from calf urine using a molecularly imprinted polymer followed by quantitation by high-performance liquid chromatography with UV detection [J].

Blomgren, A ;

Berggren, C ;

Holmberg, A ;

Larsson, F ;

Sellergren, B ;

Ensing, K .

JOURNAL OF CHROMATOGRAPHY A, 2002, 975 (01) :157-164

[3] Molecularly imprinted solid-phase extraction of naphthalene sulfonates from water [J].

Caro, E ;

Marcé, RM ;

Cormack, PAG ;

Sherrington, DC ;

Borrull, F .

JOURNAL OF CHROMATOGRAPHY A, 2004, 1047 (02) :175-180

[4]

Dandekar T., 1993, BIOCH MOL BIOL, V4, P75

[5] Evaluation of methods aimed at complete removal of template from molecularly imprinted polymers [J].

Ellwanger, A ;

Berggren, C ;

Bayoudh, S ;

Crecenzi, C ;

Karlsson, L ;

Owens, PK ;

Ensing, K ;

Cormack, P ;

Sherrington, D ;

Sellergren, B .

ANALYST, 2001, 126 (06) :784-792

[6]

EMEA, EUR AG EV MED PROD V

[7] Syntheses of molecularly imprinted polymers: Molecular recognition of cyproheptadine using original print molecules and azatadine as dummy templates [J].

Feas, X. ;

Seijas, J. A. ;

Vazquez-Tato, M. P. ;

Regal, P. ;

Cepeda, A. ;

Fente, C. .

ANALYTICA CHIMICA ACTA, 2009, 631 (02) :237-244

[8] Novel cyromazine imprinted polymer applied to the solid-phase extraction of melamine from feed and milk samples [J].

He, Limin ;

Su, Yijuan ;

Zheng, Yaqiu ;

Huang, Xianhui ;

Wu, Li ;

Liu, Yahong ;

Zeng, Zhenling ;

Chen, Zhangliu .

JOURNAL OF CHROMATOGRAPHY A, 2009, 1216 (34) :6196-6203

[9]

HENNION MC, 1994, ENVIRON SCI TECHNOL, V28, pA576, DOI 10.1021/es00062a717

[10]

Horkovics-Kovats S., 1997, Journal of Veterinary Pharmacology and Therapeutics, V20, P29

← 1 2 3 →