Effect of lignosulfonates on the dry matter loss, nutritional value, and microbial counts of high moisture alfalfa silage

被引:2
作者
Leon-Tinoco, A. Y. [1 ]
Guimaraes, B. C. [2 ]
Almeida, S. T. R. [2 ]
Reyes, D. C. [1 ]
Rivera, S. [1 ]
Killerby, M. [1 ]
Wu, C. [3 ]
Perkins, B. [4 ]
Knight, C. [5 ]
Romero, J. J. [1 ]
机构
[1] Univ Maine, Anim & Vet Sci, Orono, ME 04469 USA
[2] Univ Lavras, Dept Anim Sci, Lavras, MG, Brazil
[3] Univ Delaware, Dept Anim & Food Sci, Newark, DE 19716 USA
[4] Univ Maine, Food Sci & Human Nutr, Orono, ME 04469 USA
[5] Univ Maine Cooperat Extens, Orono, ME 04469 USA
基金
美国食品与农业研究所;
关键词
Alfalfa; Fermentation; Inoculant; Lignosulfonate; Protein fermentation; Silage; LACTIC-ACID BACTERIA; NEUTRAL DETERGENT FIBER; TREATED SOYBEAN-MEAL; CANOLA-MEAL; CALCIUM LIGNOSULFONATE; FERMENTATION QUALITY; UNDEGRADABLE PROTEIN; FORAGE; DIGESTIBILITY; DEGRADABILITY;
D O I
10.1016/j.anifeedsci.2022.115346
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
In this experiment, we evaluated the effects of sodium lignosulfonate (NaL) and magnesium lignosulfonate (MgL) applied independently at 0, 5, 10, and 15 (g/kg, fresh weight basis) and an inoculant (INO) on high-moisture (DM: 219 g/kg, fresh weight basis) alfalfa (Medicago saliva L.) silage nutrient preservation. The INO consisted of Pediococcus pentosaceus and Lactobacillus plantarum applied at a rate of 4.95 and 4.00 log cfu/g (fresh weight basis), respectively. Data were analyzed as a randomized complete block design (RCBD; 5 blocks) and linear and quadratic polynomial contrasts were used to determine dose rate effects for NaL and MgL and orthogonal contrasts for INO effects. At d 0, increasing NaL dose from 0 to 15 g/kg increased linearly DM (219-227 g/kg) and decreased linearly mold counts (4.48-3.62 log cfu/g; fresh weight basis). No treatment effects were observed on lactic acid bacteria counts (7.04 log cfu/g; fresh weight basis), water soluble carbohydrates (61.5), and aNDF (432 g/kg of DM). After 229 d of ensiling, both MgL and INO increased DM loss ((x) over bar = 13.7 and 13.7 vs 11.3% of DM) due to a lower production of lactic acid ((x) over bar = 75.5 and 78.3 vs 92.3 g/kg of DM, respectively) which resulted in a higher pH relative to untreated silage ((x) over bar = 4.41 and 4.46 vs 4.33; respectively). Increasing doses from 0 (6.42) to 15 g/kg for both NaL (6.90) and MgL (7.06 log cfu/g; fresh weight basis) resulted in linear and quadratic increases of lactic acid bacteria counts, respectively. These counts were also higher in INO treated silages (6.98 log cfu/g; fresh weight basis), relative to untreated silage. Furthermore, both NaL and MgL decreased in vitro ruminal total volatile fatty acid concentration (x = 87.0 and 93.1 vs 97.1 mM; respectively), compared with the untreated silage. The greater acidification in untreated silage prevented additives tested from reducing the generation of NH3-N ((x) over bar = 110 g/kg of N) during ensiling. Overall, none of the additives tested improved the preservation of high-moisture alfalfa silage nutrients beyond what was observed in untreated.
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页数:13
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