Determination of rutin content in tartary buckwheat (Fagopyrum Tataricum (L.) Gaertn.) by three analytical methods

Rutin is a natural antioxidant beneficial to human health. The objective of the study was to compare the results of three analytical methods (HPLC and two spectrophotometric ones) used for the determination of rutin concentration in plant material that was represented by Tartary buckwheat from different countries of origin. The highest rutin concentration was measured in leaves, followed by seeds and stems. The lowest concentrations were determined in roots. Generally, the lowest concentrations of rutin were measured in Fagopyrum tataricum (L.) Gaertn. Originating from Pakistan. A spectrophotometric method using AlCl3 is very expeditious and can be considered as suitable for routine measurements of rutin content in plant material.