Expression of Recombinant Potato leafroll virus Structural and Non-structural Proteins for Antibody Production

被引：7

作者：

Plchova, Helena ^{[1
]}

Moravec, Tomas ^{[1
]}

Dedic, Petr ^{[2
]}

Cerovska, Noemi ^{[1
]}

机构：

[1] Acad Sci Czech Republ, Inst Expt Bot, VVI, Prague 16000 6, Czech Republic

[2] Potato Res Inst, Havlickuv Brod 58001, Czech Republic

来源：

JOURNAL OF PHYTOPATHOLOGY | 2011年 / 159卷 / 02期

关键词：

Potato leafroll virus; recombinant viral antigen; antibody production; MOP-TOP-VIRUS; POLYCLONAL ANTIBODIES; COAT PROTEIN; ESCHERICHIA-COLI;

D O I：

10.1111/j.1439-0434.2010.01740.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Vector pMPM-A4 Omega and vectors pQE-30 and pET-45b(+) containing the 6x His-tag sequence were used for expression of Potato leafroll virus (PLRV) structural and non-structural proteins in Escherichia coli. Coat protein (CP) and RNA-dependent RNA polymerase (RdRp)-fragments RdRp(43-616) and RdRp(304-537) were chosen for expression. A high level of CP and RdRp(304-537) was obtained only in an expression system using pET-45b(+) vector and E. coli Rosetta-gami 2(DE3) cells. After purification, the His-tagged PLRV proteins were used for immunization of rabbits.

引用

页码：130 / 132

页数：3

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