Identification of Elm Yellows Phytoplasma in Plum Trees in China

被引：7

作者：

Hong, Min ^{[1
,2
,3
,4
]}

Zhang, Chunping ^{[1
,2
]}

Li, Zhengnan ^{[1
,2
]}

Zhang, Jue ^{[3
,4
]}

Zhao, Zhen ^{[3
,4
]}

Song, Jiagui ^{[1
,2
]}

Wu, Yunfeng ^{[1
,2
]}

机构：

[1] NW A&F Univ, Coll Plant Protect, Yangling 712100, Peoples R China

[2] NW A&F Univ, Shaanxi Key Lab Mol Biol Agr, Yangling 712100, Peoples R China

[3] NW A&F Univ, Coll Life Sci, Yangling 712100, Peoples R China

[4] NW A&F Univ, Shaanxi Key Lab Mol Biol Agr, Yangling 712100, Peoples R China

来源：

JOURNAL OF PHYTOPATHOLOGY | 2011年 / 159卷 / 01期

基金：

中国国家自然科学基金;

关键词：

plum; phytoplasma; Polymerase Chain Reaction; Restriction Fragment Length Polymorphism; sequencing; phylogenetic analyses; China; GRAPEVINE YELLOWS; CLASSIFICATION; DISEASES; RFLP; WILD;

D O I：

10.1111/j.1439-0434.2010.01722.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Plum plants (Prunus cerasifera Ehrh) with small and rolled leaves resembling symptoms of phytoplasma infection were observed during 2008 and 2009 in the ornamental garden of Northwest A&F University (Republic of China). Nested polymerase chain reaction (PCR) using a combination of phytoplasma-specific universal primer pairs (R16F2m/R16R1m-R16F2n/R16R2) amplified 16S rDNA with the expected size (1.2 kb) from all samples of symptomatic plum plants. Sequencing results and restriction fragment length polymorphism (RFLP) analysis of the 1248 bp R16F2n/R16R2 products showed that the phytoplasma belongs to group 16SrV. Phylogenetic analysis showed that the phytoplasma had a close relation to JWB phytoplasma. This is, we believe, the first report of elm yellows phytoplasma infecting plum plants in China.

引用

页码：57 / 59

页数：3

共 25 条

[1]

Angelini E, 2001, VITIS, V40, P79

[2] Role of wild Prunus species in the epidemiology of European stone fruit yellows [J].

Carraro, L ;

Ferrini, F ;

Ermacora, P ;

Loi, N .

PLANT PATHOLOGY, 2002, 51 (04) :513-517

[3] Detection and differentiation of phytoplasmas in Australia [J].

Davis, RI ;

Schneider, B ;

Gibb, KS .

AUSTRALIAN JOURNAL OF AGRICULTURAL RESEARCH, 1997, 48 (05) :535-544

[4]

DEL SP, 1998, PHYTOPATHOL MEDITERR, V37, P133

[5]

GOIDANICH G, 1933, REGIA STAZIONE PATOL, V13, P160

[6]

Gu PeiWen Gu PeiWen, 2007, Acta Phytopathologica Sinica, V37, P390

[7]

Gundersen D. E., 1996, Phytopathologia Mediterranea, V35, P144

[8] 'Candidatus Phytoplasma ziziphi', a novel phytoplasma taxon associated with jujube witches'-broom disease [J].

Jung, HY ;

Sawayanagi, T ;

Kakizawa, S ;

Nishigawa, H ;

Wei, W ;

Oshima, K ;

Miyata, S ;

Ugaki, M ;

Hibi, T ;

Namba, S .

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2003, 53 :1037-1041

[9] Classification of phytoplasma strains in the elm yellows group (16SrV) and proposal of 'Candidatus Phytoplasma ulmi' for the phytoplasma associated with elm yellows [J].

Lee, IM ;

Martini, M ;

Marcone, C ;

Zhu, SF .

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2004, 54 :337-347

[10] UNIVERSAL AMPLIFICATION AND ANALYSIS OF PATHOGEN 16S RDNA FOR CLASSIFICATION AND IDENTIFICATION OF MYCOPLASMALIKE ORGANISMS [J].

LEE, IM ;

HAMMOND, RW ;

DAVIS, RE ;

GUNDERSEN, DE .

PHYTOPATHOLOGY, 1993, 83 (08) :834-842

← 1 2 3 →