A New Electrochemical Multiplexed Assay for PSA Cancer Marker Detection

被引：55

作者：

Zani, Angela ^{[1
]}

Laschi, Serena ^{[1
]}

Mascini, Marco ^{[1
,2
]}

Marrazza, Giovanna ^{[1
]}

机构：

[1] Univ Florence, Dipartimento Chim, I-50019 Florence, Italy

[2] Ist Nazl Biostrut & Biosistemi, I-00136 Rome, Italy

来源：

ELECTROANALYSIS | 2011年 / 23卷 / 01期

关键词：

PSA; Tumour marker; Electrochemical detection; Microfluidic; Magnetic beads; Multiarray; PROSTATE-SPECIFIC ANTIGEN; MAGNETIC BEADS; SENSITIVE DETECTION; BIOSENSOR; IMMUNOASSAY; DIAGNOSIS; SYSTEM; SERUM; IMMUNOSENSOR; BIOMARKERS;

D O I：

10.1002/elan.201000486

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In this paper, two simple and sensitive approaches for prostate specific antigen (PSA) detection are presented, by using antibody modified paramagnetic microparticles coupled to multiplexed electrochemical platforms. The first proposed approach is based on 8-sensors screen-printed arrays as candidate for electrochemical transducers and a simple target capturing step by means of antibody-functionalised magnetic beads. In order to improve the performances of the immunosensing, a second approach is developed by using GRAVI-Cell. This is microfluidic-based affinity assay device for running bead-protocols. This innovative system combines a special chip containing eight polymer microchannels, with a portable, computer-controlled instrument. Both immunosensing strategies developed promise to be a sensitive, multiplexed tool for fast and easy PSA analysis.

引用

页码：91 / 99

页数：9

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