Dissection of culture media for embryos: the most important and less important components and characteristics

被引:73
作者
Gardner, David K. [1 ]
机构
[1] Univ Melbourne, Dept Zool, Parkville, Vic 3052, Australia
关键词
D O I
10.1071/RD07160
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Improvements in culture media formulations have led to an increase in the ability to maintain the mammalian embryo in culture throughout the preimplantation and pre-attachment period. Amino acids and specific macromolecules have been identified as being key medium components, whereas temporal dynamics have been recognised as important media characteristics. Furthermore, other laboratory factors that directly impact embryo development and viability have been identified. Such factors include the use of a reduced oxygen tension, an appropriate incubation system and an adequate prescreening of all contact supplies. With rigourous quality systems in place, it is possible to obtain in vivo rates of embryo development in vitro using new media formulations while maintaining high levels of embryo viability. The future of embryo culture will likely be based on novel culture chips capable of providing temporal dynamics while facilitating real-time analysis of embryo physiology.
引用
收藏
页码:9 / 18
页数:10
相关论文
共 109 条
[1]   EFFECT OF SODIUM AND BETAINE IN CULTURE MEDIA ON DEVELOPMENT AND RELATIVE RATES OF PROTEIN-SYNTHESIS IN PREIMPLANTATION MOUSE EMBRYOS INVITRO [J].
ANBARI, K ;
SCHULTZ, RM .
MOLECULAR REPRODUCTION AND DEVELOPMENT, 1993, 35 (01) :24-28
[2]   Comparison of two sequential media for culturing cleavage-stage embryos and blastocysts: embryo characteristics and clinical outcome [J].
Balaban, B ;
Urman, B .
REPRODUCTIVE BIOMEDICINE ONLINE, 2005, 10 (04) :485-491
[3]   INFLUENCE OF SINGLE AMINO-ACIDS ON THE DEVELOPMENT OF HAMSTER ONE-CELL EMBRYOS INVITRO [J].
BAVISTER, BD ;
ARLOTTO, T .
MOLECULAR REPRODUCTION AND DEVELOPMENT, 1990, 25 (01) :45-51
[4]   Recombinant human albumin supports hamster in-vitro fertilization [J].
Bavister, BD ;
Kinsey, DL ;
Lane, M ;
Gardner, DK .
HUMAN REPRODUCTION, 2003, 18 (01) :113-116
[6]   Culture of preimplantation embryos: Facts and artifacts [J].
Bavister, BD .
HUMAN REPRODUCTION UPDATE, 1995, 1 (02) :91-148
[7]  
BAVISTER BD, 1992, PREIMPLANTATION EMBR, P57
[8]  
Bavister BD, 1987, MAMMALIAN PREIMPLANT, P219
[9]   Discrepancies between the effects of glutamine in cultures of preimplantation mouse embryos [J].
Biggers, JD ;
McGinnis, LK ;
Summers, MC .
REPRODUCTIVE BIOMEDICINE ONLINE, 2004, 9 (01) :70-73
[10]   THE PROTECTIVE ACTION OF BETAINE ON THE DELETERIOUS EFFECTS OF NACL ON PREIMPLANTATION MOUSE EMBRYOS INVITRO [J].
BIGGERS, JD ;
LAWITTS, JA ;
LECHENE, CP .
MOLECULAR REPRODUCTION AND DEVELOPMENT, 1993, 34 (04) :380-390