Biomaterial topography alters healing in vivo and monocyte/macrophage activation in vitro

被引:145
作者
Bota, Paige C. S. [1 ]
Collie, Angela M. B. [1 ]
Puolakkainen, Pauli [2 ]
Vernon, Robert B. [2 ]
Sage, E. Helene [2 ]
Ratner, Buddy D. [1 ]
Stayton, Patrick S. [1 ]
机构
[1] Univ Washington, Dept Bioengn, Seattle, WA 98195 USA
[2] Benaroya Res Inst Virginia Mason, Hope Heart Program, Seattle, WA 98101 USA
基金
美国国家科学基金会;
关键词
human monocytes/macrophages; topography; surface dependent behavior; foreign body reaction; microarray; ENCAPSULATES SUBCUTANEOUS IMPLANTS; CYTOKINE MESSENGER-RNAS; MONOCYTES MACROPHAGES; BIOMEDICAL POLYMERS; SURFACE; TISSUE; GENERATION; CHEMOKINES; CELLS; QUANTIFICATION;
D O I
10.1002/jbm.a.32893
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
The effect of biomaterial topography on healing in vivo and monocyte/macrophage stimulation in vitro was assessed. A series of expanded polytetrafluoroethylene (ePTFE) materials were characterized by increasing average intranodal distance of 1.2 mu m (1.2-ePTFE), 3.0 mu m (3.0-ePTFE), and 4.4 mu m (4.4-ePTFE), but presented consistent surface chemistry with nonporous PTFE (np-PTFE). Subcutaneous implantation of 4.4-ePTFE into mice resulted in a statistically thinner capsule that appeared less organized and less dense than the np-PTFE response. In vitro, isolated monocytes/macrophages cultured on np-PTFE produced low levels of interleukin 1-beta (IL-1 beta), 1.2-ePTFE and 3.0-ePTFE stimulated intermediate levels, and 4.4-ePTFE stimulated a 15-fold increase over np-PTFE. Analysis of cDNA microarrays demonstrated that additional proinflammatory cytokines and chemokines, including IL-1 beta, interleukin 6, tumor necrosis factor alpha, monocyte chemotactic protein 1, and macrophage inflammatory protein 1-beta, were expressed at higher levels by monocytes/macrophages cultured on 4.4-ePTFE at 4 and 24 h, respectively. Expression ratios for several genes were quantified by RT-PCR and were consistent with those from the cDNA array results. These results demonstrate the effect of biomaterial topography on early proinflammatory cytokine production and gene transcription by monocytes/macrophages in vitro and decreased fibrous capsule thickness in vivo. (C) 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 95A: 649-657, 2010.
引用
收藏
页码:649 / 657
页数:9
相关论文
共 33 条
[1]   Biological responses to materials [J].
Anderson, JM .
ANNUAL REVIEW OF MATERIALS RESEARCH, 2001, 31 :81-110
[2]   Adherence regulates macrophage signal transduction and primes tumor necrosis factor production [J].
Bauer, GJ ;
Arbabi, S ;
Garcia, IA ;
deHingh, I ;
Rosengart, MR ;
Maier, RV .
SHOCK, 2000, 14 (04) :435-440
[3]   CYTOKINE AND GROWTH-FACTOR PRODUCTION BY MONOCYTES MACROPHAGES ON PROTEIN PREADSORBED POLYMERS [J].
BONFIELD, TL ;
COLTON, E ;
MARCHANT, RE ;
ANDERSON, JM .
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH, 1992, 26 (07) :837-850
[4]   FUNCTIONAL VERSUS QUANTITATIVE COMPARISON OF IL-1-BETA FROM MONOCYTES MACROPHAGES ON BIOMEDICAL POLYMERS [J].
BONFIELD, TL ;
ANDERSON, JM .
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH, 1993, 27 (09) :1195-1199
[5]   NEOVASCULARIZATION OF SYNTHETIC MEMBRANES DIRECTED BY MEMBRANE MICROARCHITECTURE [J].
BRAUKER, JH ;
CARRBRENDEL, VE ;
MARTINSON, LA ;
CRUDELE, J ;
JOHNSTON, WD ;
JOHNSON, RC .
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH, 1995, 29 (12) :1517-1524
[6]   Low copy number detection of herpes simplex virus type 1 mRNA and mouse Th1 type cytokine mRNAs by Light Cycler quantitative real-time PCR [J].
Broberg, EK ;
Nygårdas, M ;
Salmi, AA ;
Hukkanen, V .
JOURNAL OF VIROLOGICAL METHODS, 2003, 112 (1-2) :53-65
[7]   In vivo leukocyte cytokine mRNA responses to biomaterials are dependent on surface chemistry [J].
Brodbeck, WG ;
Voskerician, G ;
Ziats, NP ;
Nakayama, Y ;
Matsuda, T ;
Anderson, JM .
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 2003, 64A (02) :320-329
[8]  
Campbell C E, 1989, J Invest Surg, V2, P51, DOI 10.3109/08941938909016503
[9]   TNF AND IL-1 GENERATION BY HUMAN MONOCYTES IN RESPONSE TO BIOMATERIALS [J].
CARDONA, MA ;
SIMMONS, RL ;
KAPLAN, SS .
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH, 1992, 26 (07) :851-859
[10]  
Crowther M, 2001, J LEUKOCYTE BIOL, V70, P478