Effect of ischemia-reperfusion on the renal brush-border membrane sodium-dependent phosphate cotransporter NaPi-2

被引:7
|
作者
Xiao, Y [1 ]
Desrosiers, RR [1 ]
Béliveau, R [1 ]
机构
[1] Univ Quebec, Hop St Justine, Mol Med Lab, CP 8888, Montreal, PQ H3C 3P8, Canada
关键词
kidney; ischemia; reperfusion; phosphate; transport;
D O I
10.1139/y00-122
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
To understand the mechanisms underlying ischemia-reperfusion-induced renal proximal tubule damage, we analyzed the expression of the Na+-dependent phosphate (Na+/P-i) cotransporter NaPi-2 in brush border membranes (BBM) isolated from rats which had been subjected to 30 min renal ischemia and 60 min reperfusion. Na+/P-i cotransport activities of the BBM vesicles were also determined. Ischemia caused a significant decrease (about 40%, P < 0.05) in all forms of NaPi-2 in the BBM, despite a significant increase (31 +/- 3%, P < 0.05) in the Na+/P-i cotransport activity. After reperfusion, both NaPi-2 expression and Na+/P-i cotransport activity returned to control levels. In contrast with Na+/P-i cotransport, ischemia significantly decreased Na+-dependent glucose cotransport but did not affect Na+-dependent proline cotransport. Reperfusion caused further decreases in both Na+/glucose (by 60%) and Na+/proline (by 33%) cotransport. Levels of NaPi-2 were more reduced in the BBM than in cortex homogenates, suggesting a relocalization of NaPi-2 as a result of ischemia. After reperfusion, NaPi-2 levels returned to control values in both BBM and homogenates. These data indicate that the NaPi-2 protein and BBM Na+/P-i cotransport activity respond uniquely to reversible renal ischemia and reperfusion, and thus may play an important role in maintaining and restoring the structure and function of the proximal tubule.
引用
收藏
页码:206 / 212
页数:7
相关论文
共 24 条
  • [1] Effect of ischemia reperfusion on sodium-dependent phosphate transport in renal brush border membranes
    Khundmiri, SJ
    Asghar, M
    Banday, AA
    Khan, F
    Salim, S
    Levi, M
    Yusufi, ANK
    BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 2005, 1716 (01): : 19 - 28
  • [2] Acute regulation by dietary phosphate of the sodium-dependent phosphate transporter (NaPi-2) in rat kidney
    Katai, K
    Segawa, H
    Haga, H
    Morita, K
    Arai, H
    Tatsumi, S
    Taketani, Y
    Miyamoto, K
    Hisano, S
    Fukui, Y
    Takeda, E
    JOURNAL OF BIOCHEMISTRY, 1997, 121 (01): : 50 - 55
  • [3] Phosphate deprivation induces overexpression of two proteins related to the rat renal phosphate cotransporter NaPi-2
    Boyer, CJC
    Xiao, YS
    Dugre, A
    Vincent, E
    Delisle, MC
    Beliveau, R
    BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 1996, 1281 (01): : 117 - 123
  • [4] THIOL REDOX AND PHOSPHATE-TRANSPORT IN RENAL BRUSH-BORDER MEMBRANE - EFFECT OF NICOTINAMIDE
    SUZUKI, M
    CAPPARELLI, AW
    JO, OD
    YANAGAWA, N
    BIOCHIMICA ET BIOPHYSICA ACTA, 1990, 1021 (01) : 85 - 90
  • [5] OLIGOMERIC STRUCTURE OF THE SODIUM-DEPENDENT PHLORHIZIN BINDING-PROTEIN FROM KIDNEY BRUSH-BORDER MEMBRANES
    GERARDILAFFIN, C
    DELQUEBAYER, P
    SUDAKA, P
    POIREE, JC
    BIOCHIMICA ET BIOPHYSICA ACTA, 1993, 1151 (01) : 99 - 104
  • [6] SODIUM-TAURINE COTRANSPORT IN REPTILIAN RENAL BRUSH-BORDER MEMBRANE-VESICLES
    BENYAJATI, S
    BAY, SM
    PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 1992, 421 (2-3): : 168 - 175
  • [7] Inorganic phosphate homeostasis in sodium-dependent phosphate cotransporter Npt2b+/- mice
    Ohi, Akiko
    Hanabusa, Etsuyo
    Ueda, Otoya
    Segawa, Hiroko
    Horiba, Naoshi
    Kaneko, Ichiro
    Kuwahara, Shoji
    Mukai, Tomo
    Sasaki, Shohei
    Tominaga, Rieko
    Furutani, Junya
    Aranami, Fumito
    Ohtomo, Shuichi
    Oikawa, Yumiko
    Kawase, Yousuke
    Wada, Naoko A.
    Tachibe, Takanori
    Kakefuda, Mami
    Tateishi, Hiromi
    Matsumoto, Kaoru
    Tatsumi, Sawako
    Kido, Shinsuke
    Fukushima, Naoshi
    Jishage, Kou-ichi
    Miyamoto, Ken-ichi
    AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY, 2011, 301 (05) : F1105 - F1113
  • [8] Downregulation of renal type IIa sodium-dependent phosphate cotransporter during lipopolysaccharide-induced acute inflammation
    Ikeda, Shoko
    Yamamoto, Hironori
    Masuda, Masashi
    Takei, Yuichiro
    Nakahashi, Otoki
    Kozai, Mina
    Tanaka, Sarasa
    Nakao, Mari
    Taketani, Yutaka
    Segawa, Hiroko
    Iwano, Masayuki
    Miyamoto, Ken-ichi
    Takeda, Eiji
    AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY, 2014, 306 (07) : F744 - F750
  • [9] PARAQUAT(2+)/H+ EXCHANGE IN ISOLATED RENAL BRUSH-BORDER MEMBRANE-VESICLES
    WRIGHT, SH
    WUNZ, TM
    BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 1995, 1240 (01): : 18 - 24
  • [10] Protective Effect of Nicotinamide Adenine Dinucleotide Phosphate on Renal Ischemia-Reperfusion Injury
    Weng, Xiao-fen
    Li, Song-tao
    Song, Qi
    Zhu, Qi
    Song, Dan-dan
    Qin, Zheng-hong
    Xie, Yan
    KIDNEY & BLOOD PRESSURE RESEARCH, 2018, 43 (03): : 651 - 663