Antioxidant and antibacterial activities of various seabuckthorn (Hippophae rhamnoides L.) seed extracts

Seabuckthorn (Hippophae rhamnoides L.) seeds were successively extracted with chloroform, ethyl acetate, acetone and methanol (MeOH) using a Soxhlet extractor for 8 It each. The crude extracts were screened for antioxidant and antibacterial activities. The reducing power and antioxidant activities evaluated in various in vitro models (1, 1-diphenyl-2-picrylhydrazine and liposome model system) showed the highest activity for MeOH extract. The MeOH extract was also found to posses maximum antibacterial activity. The MIC values, with respect to MeOH extract for Bacillus cereus, Bacillus coagulans, Bacillus subtilis, Listeria monocytogenes, Yersinia enterocolitica, were found to be 200, 300, 300, 300, and 350 ppm, respectively. These results indicated the possibility of using seabuckthorn seeds for medicinal uses and food preservation. (c) 2004 Elsevier Ltd. All rights reserved.