Thermotolerant Oil-Degrading Bacteria Isolated from Soil and Water of Geographically Distant Regions

被引：4

作者：

Delegan, Ya. A. ^{[1
,2
]}

Vetrova, A. A. ^{[2
]}

Akimov, V. N. ^{[2
]}

Titok, M. A. ^{[3
]}

Filonov, A. E. ^{[1
,2
]}

Boronin, A. M. ^{[2
]}

机构：

[1] Pushchino State Inst Nat Sci, Pushchino 142290, Moscow Oblast, Russia

[2] Russian Acad Sci, Skryabin Inst Biochem & Physiol Microorganisms, Pushchino 142290, Moscow Oblast, Russia

[3] Belarusian State Univ, Minsk 220030, BELARUS

来源：

APPLIED BIOCHEMISTRY AND MICROBIOLOGY | 2016年 / 52卷 / 04期

基金：

俄罗斯基础研究基金会;

关键词：

bioremediation; biodegradation; crude oil; oil-degrading bacteria; thermotolerant bacteria; Gordonia; Rhodococcus; SP NOV; PHYLOGENETIC ANALYSIS; GENES; IDENTIFICATION; PRIMERS; ALKANE; GYRB;

D O I：

10.1134/S0003683816040025

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Oil-degrading bacteria were isolated from soil and water samples taken in Russia, Kazakhstan, and the Antarctic; 13 of 86 strains proved to be thermotolerant. These bacteria utilized crude oil at 45-50 degrees C; their growth optimum (35-37 degrees C) and range (20-53 degrees C) differ from those of mesophilic bacteria. Thermotolerant strains were identified as representatives of the genera Rhodococcus and Gordonia. It was shown that their ability to degrade petroleum products does not differ at 24 and 45 degrees C. The strains Rhodococcus sp. Par7 and Gordonia sp. 1D utilized 14 and 20% of the oil, respectively, in 14 days at 45 degrees C. All of the isolated thermotolerant bacteria grew in a medium containing 3% NaCl; the medium for the strains Gordonia amicalis 1B and Gordonia sp. 1D contained up to 10% NaCl. The bacteria G. amicalis and Rhodococcus erythropolis were able to utilize crude oil and individual hydrocarbons at higher (up to 50 degrees C) temperatures.

引用

页码：389 / 396

页数：8

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