Acoustic Ejection Mass Spectrometry for High-Throughput Analysis

被引：61

作者：

Zhang, Hui ^{[1
]}

Liu, Chang ^{[3
]}

Hua, Wenyi ^{[1
]}

Ghislain, Lucien P. ^{[2
]}

Liu, Jianhua ^{[1
]}

Aschenbrenner, Lisa ^{[1
]}

Noell, Stephen ^{[1
]}

Dirico, Kenneth J. ^{[1
]}

Lanyon, Lorraine F. ^{[1
]}

Steppan, Claire M. ^{[1
]}

West, Mike ^{[1
]}

Arnold, Don W. ^{[3
,4
]}

Covey, Thomas R. ^{[3
]}

Datwani, Sammy S. ^{[2
]}

Troutman, Matthew D. ^{[1
]}

机构：

[1] Pfizer Global Res & Dev, Groton, CT 06340 USA

[2] Beckman Coulter Life Sci Inc, San Jose, CA 95134 USA

[3] SCIEX Ltd, Concord, ON L4K 4V8, Canada

[4] Veristad LLC, Livermore, CA 94550 USA

来源：

ANALYTICAL CHEMISTRY | 2021年 / 93卷 / 31期

关键词：

PORT SAMPLING INTERFACE; FLUORESCENCE READOUTS; IONIZATION; PROBE;

D O I：

10.1021/acs.analchem.1c01137

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We describe a mass spectrometry (MS) analytical platform resulting from the novel integration of acoustic droplet ejection (ADE) technology, an open-port interface (OPI), and electrospray ionization (ESI)-MS that creates a transformative system enabling high-speed sampling and label-free analysis. The ADE technology delivers nanoliter droplets in a touchless manner with high speed, precision, and accuracy. Subsequent sample dilution within the OPI, in concert with the capabilities of modern ESI-MS, eliminates the laborious sample preparation and method development required in current approaches. This platform is applied to a variety of experiments, including high-throughput (HT) pharmacology screening, label-free in situ enzyme kinetics, in vitro absorption, distribution, metabolism, elimination, pharmacokinetic and biomarker analysis, and HT parallel medicinal chemistry.

引用

页码：10850 / 10861

页数：12

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