Jiedu Sangen Decoction Inhibits Migration and Invasion of Colon Cancer SW480 Cells via Suppressing Epithelial Mesenchymal Transition

被引：9

作者：

Zhang, Kai ^{[1
,2
]}

Peng, Tao ^{[3
]}

Yan, Qingying ^{[1
]}

Sun, Leitao ^{[1
]}

Miao, Haojun ^{[1
]}

Yuan, Li ^{[1
]}

Hong, Yuxin ^{[1
]}

Zhou, Mengmeng ^{[1
]}

Guo, Kaibo ^{[1
]}

Zhang, Leyin ^{[1
]}

Fang, Jing ^{[4
]}

Shen, Minhe ^{[5
]}

Ruan, Shanming ^{[5
]}

机构：

[1] Zhejiang Chinese Med Univ, Clin Med Coll 1, Hangzhou 310053, Zhejiang, Peoples R China

[2] Mem Sloan Kettering Canc Ctr, Human Oncol & Pathogenesis Program, New York, NY 10065 USA

[3] Shaoxing City Keqiao Dist Hosp Tradit Chinese Med, Dept Oncol, Shaoxing 312030, Peoples R China

[4] First Peoples Hosp Yuhang Dist Hangzhou, Dept Tradit Chinese Internal Med, Hangzhou 310000, Zhejiang, Peoples R China

[5] Zhejiang Chinese Med Univ, Affiliated Hosp 1, Dept Med Oncol, Hangzhou 310006, Zhejiang, Peoples R China

来源：

EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE | 2018年 / 2018卷

基金：

中国博士后科学基金; 中国国家自然科学基金;

关键词：

N-CADHERIN; COLORECTAL-CANCER; DOWN-REGULATION; STEM-CELLS; CARCINOMA; METASTASIS; EXPRESSION; TWIST; STATISTICS; MECHANISMS;

D O I：

10.1155/2018/1495768

中图分类号：

R [医药、卫生];

学科分类号：

10 ;

摘要：

Jiedu Sangen Decoction (JSD), a traditional Chinese medicine (TCM) formula, has been widely used in China to treat gastrointestinal cancer, especially as an adjuvant therapy in colorectal cancer (CRC) patients. This study aimed to evaluate the efficacy of JSD and Jiedu Sangen aqueous extract (JSAE) in colon cancer cells and explored the underlining mechanisms by cytotoxicity assay, scratch assay, transwell migration assay, mat rigel invasion assay, confocal laser scanning microscopy, and western blot analysis. We demonstrated that JSAE inhibited the growth of colon cancer SW480 cells in a dose-dependent manner and J SAE repressed cancer cell migration and invasion. Furthermore, epithelial mesenchymal transition (EMT) was reversed by JSAE via enhancing E-cadherin expression and attenuating protein levels of EMT promoting factors such as N-cadherin, Slug, and ZEB1. These findings provided the first experimental evidence confirming the efficacy of JSAE in repressing invasion and metastasis of CRC and paving a way for the broader use of JSD in clinic.

引用

页数：10

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