The Contribution of the Tie2+ Lineage to Primitive and Definitive Hematopoietic Cells

被引:146
作者
Tang, Yuefeng [1 ]
Harrington, Anne [1 ]
Yang, Xuehui [1 ]
Friesel, Robert E. [1 ]
Liaw, Lucy [1 ]
机构
[1] Maine Med Ctr, Res Inst, Ctr Mol Med, Scarborough, ME 04074 USA
关键词
Tie2; Cre recombinase; hematopoietic lineage; mouse transgenic; ROSA reporter; RECEPTOR TYROSINE KINASES; YOLK-SAC; HAEMOGENIC ENDOTHELIUM; MOUSE EMBRYO; STEM-CELLS; PRECURSORS; ADULT; RUNX1;
D O I
10.1002/dvg.20654
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The regulatory elements of the Tie2/Tek promoter are commonly used in mouse models to direct transgene expression to endothelial cells. Tunica intima endothelial kinase 2 (Tie2) is also expressed in hematopoietic cells, although this has not been fully characterized. We determine the lineages of adult hematopoietic cells derived from Tie2-expressing populations using Tie2-Cre;Rosa26R-EYFP mice. In Tie2-Cre;Rosa26-REYFP mice, analysis of bone marrow cells showed Cre-mediated recombination in 85% of the population. In adult bone marrow and spleen, we analyzed subclasses of early hematopoietic progenitors, T cells, monocytes, granulocytes, and B cells. We found that similar to 84% of each lineage was EYFP+, and nearly all cells that come from Tie2-expressing lineages are CD45(+), confirming widespread contribution to definitive hematopoietic cells. In addition, more than 82% of blood cells within the embryonic yolk sac were of Tie2(+) origin. Our findings of high levels of Tie2-Cre recombination in the hematopoietic lineage have implications for the use of the Tie2-Cre mouse as a lineage-restricted driver strain. genesis 48:563-567, 2010. (C) 2010 Wiley-Liss, Inc.
引用
收藏
页码:563 / 567
页数:5
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