Electrochemical Imaging of Dopamine Release from Three-Dimensional-Cultured PC12 Cells Using Large-Scale Integration-Based Amperometric Sensors

被引：65

作者：

Abe, Hiroya ^{[1
]}

Ino, Kosuke ^{[1
]}

Li, Chen-Zhong ^{[2
,3
]}

Kanno, Yusuke ^{[1
]}

Inoue, Kumi Y. ^{[1
]}

Suda, Atsushi ^{[4
]}

Kunikata, Ryota ^{[4
]}

Matsudaira, Masahki ^{[5
]}

Takahashi, Yasufumi ^{[1
,3
,6
]}

Shiku, Hitoshi ^{[1
]}

Matsue, Tomokazu ^{[1
,3
]}

机构：

[1] Tohoku Univ, Grad Sch Environm Studies, Aoba Ku, Sendai, Miyagi 9808579, Japan

[2] Florida Int Univ, Dept Biomed Engn, Nanobioengn Nanobioelect Lab, Miami, FL 33174 USA

[3] Tohoku Univ, WPI Adv Inst Mat Res, Aoba Ku, Sendai, Miyagi 9808577, Japan

[4] Japan Aviat Elect Ind Ltd, Akishima, Tokyo 1968555, Japan

[5] Tohoku Univ, Micro Syst Integrat Ctr, Aoba Ku, Sendai, Miyagi 9800845, Japan

[6] JST, PRESTO, Kawaguchi, Saitama 3320012, Japan

来源：

ANALYTICAL CHEMISTRY | 2015年 / 87卷 / 12期

基金：

日本学术振兴会; 日本科学技术振兴机构;

关键词：

SINGLE VESICLE RELEASE; EMBRYONIC STEM-CELLS; IN-VITRO; CATECHOLAMINE SECRETION; PARKINSONS-DISEASE; QUANTAL SIZE; ES CELLS; ARRAY; MICROELECTRODES; ELECTRODES;

D O I：

10.1021/acs.analchem.5b01307

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In the present study, we used a large-scale integration (LSI)-based amperometric sensor array system, designated Bio-LSI, to image dopamine release from three-dimensional (3D)-cultured PC12 cells (PC12 spheroids). The Bio-LSI device consists of 400 sensor electrodes with a pitch of 250 mu m for rapid electroehemical imaging of large, areas. PC12 spheroids were stimulated with K+ to release dopamine. Poststimulation dopai-nine release fibril the PC12 spheroids was electrochemically imaged using the Bio-LSI device. Bia-LSI clearly showed the effects of the dopaminergic drugs L-3,4-dihydroxyphenylalanine (L-DOPA) and reserpine on K+-stimulated dopamine release from PC12 spheroids. Out results demonstrate that dopamine release from PC12 spheroids can be monitored using the device, suggesting that the Bio-LSI is a promising tool for use in evaluating 3D-cultured dopaminergic cells and the effects of dopaminergic drugs. To the best of our knowledge, this report is the first to describe electrochemical imaging of dopamine release by PC12 spheroids using LSI-based amperometric sensors.

引用

页码：6364 / 6370

页数：7

共 39 条

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