An improved medium for growing Staphylococcus aureus biofilm

被引:38
作者
Chen, Ping [1 ]
Abercrombie, Johnathan J. [1 ]
Jeffrey, Nicole R. [1 ]
Leung, Kai P. [1 ]
机构
[1] USA Dent & Trauma Res Detachment, Microbiol Branch, Inst Surg Res, Ft Sam Houston, TX 78234 USA
关键词
Staphylococcus aureus; Biofilm; Human plasma; Microfluidic;
D O I
10.1016/j.mimet.2012.04.009
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A medium (Brain Heart Infusion plus 10% human plasma) was developed, tested, and validated for growing Staphylococcus aureus biofilm in vitro. With this medium, S. aureus forms reproducible and robust biofilms in flow chambers under controlled shear flow and with increased viability recovery in static well plates. (C) 2012 Published by Elsevier B.V.
引用
收藏
页码:115 / 118
页数:4
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