Expression, purification, crystallization and preliminary crystallographic analysis of the phosphoglycerate kinase from Acinetobacter baumannii

被引：1

作者：

Baretta, Kayla ^{[1
]}

Garen, Craig ^{[1
]}

Yin, Jiang ^{[1
]}

James, Michael N. G. ^{[1
]}

机构：

[1] Univ Alberta, Dept Biochem, Edmonton, AB T6G 2H7, Canada

来源：

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2012年 / 68卷

关键词：

X-RAY-DIFFRACTION; RESISTANT; PATHOGEN; SEQUENCE; YEAST; DRUGS;

D O I：

10.1107/S1744309112020222

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Acinetobacter baumannii is a common multidrug-resistant clinical pathogen that is often found in hospitals. The A. baumannii phosphoglycerate kinase (AbPGK) is involved in the key energy-producing pathway of glycolysis and presents a potential target for antibiotic development. AbPGK has been expressed and purified; it was crystallized using lithium sulfate as the precipitant. The AbPGK crystals belonged to space group P222(1). They diffracted to a resolution of 2.5 angstrom using synchrotron radiation at the Canadian Light Source.

引用

页码：790 / 792

页数：3

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