The putative gene for the first enzyme of glutathione biosynthesis in Plasmodium berghei and Plasmodium falciparum

被引：13

作者：

Birago, C ^{[1
]}

Pace, T ^{[1
]}

Picci, L ^{[1
]}

Pizzi, E ^{[1
]}

Scotti, R ^{[1
]}

Ponzi, M ^{[1
]}

机构：

[1] Ist Super Sanita, Lab Biol Cellulare, I-00161 Rome, Italy

来源：

MOLECULAR AND BIOCHEMICAL PARASITOLOGY | 1999年 / 99卷 / 01期

关键词：

Plasmodium; gamma-glutamylcysteine synthetase; glutathione biosynthesis;

D O I：

10.1016/S0166-6851(98)00179-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The putative gene for gamma-glutamylcysteine synthetase, the rate-limiting enzyme in glutathione biosynthesis, has been characterized both in Plasmodium berghei and Plasmodium falciparum. Protein sequence comparison between these two species reveals large conserved regions sharing more than 80% similarity, separated by less conserved portions. When the comparison is extended to known gamma-glutamylcysteine synthetases from other eukaryotes, a number of high similarity blocks are observed which may help in identifying sequence essential for protein function. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：33 / 40

页数：8

共 23 条

[1] BASIC LOCAL ALIGNMENT SEARCH TOOL [J].

ALTSCHUL, SF ;

GISH, W ;

MILLER, W ;

MYERS, EW ;

LIPMAN, DJ .

JOURNAL OF MOLECULAR BIOLOGY, 1990, 215 (03) :403-410

[2]

[Anonymous], 1988, Antibodies: A Laboratory Manual

[3] The malaria parasite supplies glutathione to its host cell -: Investigation of glutathione transport and metabolism in human erythrocytes infected with Plasmodium falciparum [J].

Atamna, H ;

Ginsburg, H .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1997, 250 (03) :670-679

[4] A chromatin-associated protein is encoded in a genomic region highly conserved in the Plasmodium genus [J].

Birago, C ;

Pace, T ;

Barca, S ;

Picci, L ;

Ponzi, M .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1996, 80 (02) :193-202

[5] MECHANISMS OF THE INHIBITION OF REVERSE TRANSCRIPTION BY ANTISENSE OLIGONUCLEOTIDES [J].

BOIZIAU, C ;

THUONG, NT ;

TOULME, JJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (02) :768-772

[6] PLASMODIUM-BERGHEI - IMPLICATION OF INTRACELLULAR GLUTATHIONE AND ITS RELATED ENZYME IN CHLOROQUINE RESISTANCE IN-VIVO [J].

DUBOIS, VL ;

PLATEL, DFN ;

PAULY, G ;

TRIBOULEYDURET, J .

EXPERIMENTAL PARASITOLOGY, 1995, 81 (01) :117-124

[7] CLONING AND NUCLEOTIDE-SEQUENCE OF A FULL-LENGTH CDNA FOR HUMAN LIVER GAMMA-GLUTAMYLCYSTEINE SYNTHETASE [J].

GIPP, JJ ;

CHANG, CS ;

MULCAHY, RT .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1992, 185 (01) :29-35

[8] Co-amplification of the gamma-glutamylcysteine synthetase gene gsh1 and of the ABC transporter gene pgpA in arsenite-resistant Leishmania tarentolae [J].

Grondin, K ;

Haimeur, A ;

Mukhopadhyay, R ;

Rosen, BP ;

Ouellette, M .

EMBO JOURNAL, 1997, 16 (11) :3057-3065

[9]

HENIKOFF S, 1995, GENE, V163, P17

[10] CLUSTAL - A PACKAGE FOR PERFORMING MULTIPLE SEQUENCE ALIGNMENT ON A MICROCOMPUTER [J].

HIGGINS, DG ;

SHARP, PM .

GENE, 1988, 73 (01) :237-244

← 1 2 3 →