Novel DNA methylation biomarkers for hexavalent chromium exposure: an epigenome-wide analysis

被引：13

作者：

Feng, Lingfang ^{[1
]}

Guo, Xinnian ^{[1
]}

Li, Tao ^{[1
]}

Yao, Chunji ^{[2
]}

Xia, Hailing ^{[1
]}

Jiang, Zhaoqiang ^{[1
]}

Jia, Junlin ^{[3
]}

Fang, Yuan ^{[1
]}

Shi, Li ^{[1
]}

Lu, Chensheng Alex ^{[4
]}

Lou, Jianlin ^{[1
]}

机构：

[1] Zhejiang Acad Med Sci, Inst Occupat Dis, Hangzhou 310013, Peoples R China

[2] Zhejiang Acad Med Sci, Inst Hyg, Hangzhou 310013, Peoples R China

[3] Zhejiang Univ, Sch Med, Affiliated Hosp 2, Ctr Biostatist Bioinformat & Big Data, Hangzhou 310009, Peoples R China

[4] Harvard TH Chan Sch Publ Hlth, Dept Environm Hlth, Boston, MA 02115 USA

来源：

EPIGENOMICS | 2020年 / 12卷 / 03期

基金：

中国国家自然科学基金;

关键词：

Cr(VI)-exposed populations; DNA methylation; gene expression; Illumina; 450K; targeted-bisulfite sequencing; LUNG-CANCER; POTASSIUM DICHROMATE; EXPRESSION; WORKERS; GENE; HYPOMETHYLATION; MARKERS; NICKEL; IMPACT; DAMAGE;

D O I：

10.2217/epi-2019-0216

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Aim: We aimed to identify differential methylation of genes that could illuminate the biological mechanisms of chromium (VI) toxicity in this exposure-control study. Materials & methods: DNA methylation was measured in blood samples collected from electroplating workers and controls using a combination of Infinium Methylation 450K Chip and targeted-bisulfite sequencing. QuantiGene assay was used to detect the mRNA expression of differentially methylated genes. Inductively coupled plasma-mass spectrometry was used to quantify metals in blood and urine samples. The cytosine-phosphate-guanine sites methylation and gene expression were confirmed in a human lymphoblastoid cell line. Results & conclusion: A total of 131 differentially methylated cytosine-phosphate-guanine sites were found between exposures and controls. DNA methylation of SEMA4B may serve as a potential biomarker for chromium (VI) exposure.

引用

页码：221 / 233

页数：13

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