mRNA expression of Bcl-2, Bax, caspase-3 and -7 cannot be used as a marker for apoptosis in bovine blastocysts

被引：41
作者：
Vandaele, L. [1 ]
Goossens, K. [2 ]
Peelman, L. [2 ]
Van Soom, A. [1 ]
机构：
[1] Univ Ghent, Dept Reprod Obstet & Herd Hlth, Fac Vet Med, B-9820 Merelbeke, Belgium
[2] Univ Ghent, Dept Nutr Genet & Ethol, Fac Vet Med, B-9820 Merelbeke, Belgium
来源：
ANIMAL REPRODUCTION SCIENCE | 2008年 / 106卷 / 1-2期
关键词：
apoptosis; Bcl-family; bovine; caspase; embryos; RT-PCR;
D O I：
10.1016/j.anireprosci.2007.12.016
中图分类号：
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号：
0905 ;
摘要：
At present the most widely used technique for apoptosis detection in embryos remains the in situ visualization of DNA fragmentation by terminal deoxynucleotidyl transferase-dUTP nick end labelling (TUNEL) assay although this technique may be prone to artefacts. The aim of the present study was to investigate if the mRNA expression of a set of genes involved in apoptosis (Bax, Bcl-2, caspase-3 and -7) at an earlier point in the apoptotic cascade could be a good marker for apoptosis in in vitro produced bovine embryos. After normalization to the geometric mean of three reference genes, GAPD, YWHAZ and SDHA, mRNA expression levels of Bax, Bcl-2, caspase-3 and -7 were compared in embryos treated with an apoptosis inducer, staurosporine and in non-treated embryos. None of the genes were differently expressed in treated in comparison with non-treated embryos. In conclusion, mRNA expression of Bax, Bcl-2, caspase-3 and-7 cannot be used as a reliable apoptosis detection method. Immunofluorescent staining of caspase-3 and -7 is a better choice where as for Bcl-2 no reliable and practicable alternative is available at the moment. (c) 2007 Elsevier B.V. All rights reserved.
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页码：168 / 173
页数：6
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