Single-molecule observation of protein-protein interactions in the chaperonin system

被引：88
作者：
Taguchi, H
Ueno, T
Tadakuma, H
Yoshida, M
Funatsu, T
机构：
[1] Tokyo Inst Technol, Chem Resources Lab, Yokohama, Kanagawa 2268503, Japan
[2] Waseda Univ, Sch Sci & Engn, Dept Phys, Tokyo 1698555, Japan
来源：
NATURE BIOTECHNOLOGY | 2001年 / 19卷 / 09期
关键词：
D O I：
10.1038/nbt0901-861
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
We have analyzed the dynamics of the chaperonin (GroEL)-cochaperonin (GroES) interaction at the single-molecule level. In the presence of ATP and non-native protein, binding of GroES to the immobilized GroEL occurred at a rate that is consistent with bulk kinetics measurements. However, the release of GroES from GroEL occurred after a lag period (similar to3 s) that was not recognized in earlier bulk-phase studies. This observation suggests a new kinetic intermediate in the GroEL-GroES reaction pathway.
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页码：861 / 865
页数：5
相关论文
共 37 条
[1]   Calorimetric observation of a GroEL-protein binding reaction with little contribution of hydrophobic interaction [J].
Aoki, K ;
Taguchi, H ;
Shindo, Y ;
Yoshida, M ;
Ogasahara, K ;
Yutani, K ;
Tanaka, N .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (51) :32158-32162
[2]   GroEL binds artificial proteins with random sequences [J].
Aoki, K ;
Motojima, F ;
Taguchi, H ;
Yomo, T ;
Yoshida, M .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (18) :13755-13758
[3]   The Hsp70 and Hsp60 chaperone machines [J].
Bukau, B ;
Horwich, AL .
CELL, 1998, 92 (03) :351-366
[4]   THE ORIGINS AND CONSEQUENCES OF ASYMMETRY IN THE CHAPERONIN REACTION CYCLE [J].
BURSTON, SG ;
RANSON, NA ;
CLARKE, AR .
JOURNAL OF MOLECULAR BIOLOGY, 1995, 249 (01) :138-152
[5]   A kinetic analysis of the nucleotide-induced allosteric transitions of GroEL [J].
Cliff, MJ ;
Kad, NM ;
Hay, N ;
Lund, PA ;
Webb, MR ;
Burston, SG ;
Clarke, AR .
JOURNAL OF MOLECULAR BIOLOGY, 1999, 293 (03) :667-684
[6]   Single-molecule study of transcriptional pausing and arrest by E-coli RNA polymerase [J].
Davenport, RJ ;
Wuite, GJL ;
Landick, R ;
Bustamante, C .
SCIENCE, 2000, 287 (5462) :2497-2500
[7]   IMAGING OF SINGLE FLUORESCENT MOLECULES AND INDIVIDUAL ATP TURNOVERS BY SINGLE MYOSIN MOLECULES IN AQUEOUS-SOLUTION [J].
FUNATSU, T ;
HARADA, Y ;
TOKUNAGA, M ;
SAITO, K ;
YANAGIDA, T .
NATURE, 1995, 374 (6522) :555-559
[8]  
Harada Y, 1998, METHOD CELL BIOL, V55, P117
[9]   Single-molecule imaging of RNA polymerase-DNA interactions in real time [J].
Harada, Y ;
Funatsu, T ;
Murakami, K ;
Nonoyama, Y ;
Ishihama, A ;
Yanagida, T .
BIOPHYSICAL JOURNAL, 1999, 76 (02) :709-715
[10]   ASYMMETRICAL INTERACTION OF GROEL AND GROES IN THE ATPASE CYCLE OF ASSISTED PROTEIN-FOLDING [J].
HAYERHARTL, MK ;
MARTIN, J ;
HARTL, FU .
SCIENCE, 1995, 269 (5225) :836-841
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