Evaluation of the GenoType® MTBDRsl assay for susceptibility testing of second-line anti-tuberculosis drugs

被引：30

作者：

Said, H. M. ^{[1
]}

Kock, M. M. ^{[1
,2
]}

Ismail, N. A. ^{[1
,2
]}

Baba, K. ^{[1
,2
]}

Omar, S. V. ^{[1
]}

Osman, A. G. ^{[1
]}

Hoosen, A. A. ^{[1
,2
]}

Ehlers, M. M. ^{[1
,2
]}

机构：

[1] Univ Pretoria, Fac Hlth Sci, Dept Med Microbiol, ZA-0002 Pretoria, South Africa

[2] Tshwane Acad Div, Natl Hlth Lab Serv, Pretoria, South Africa

来源：

INTERNATIONAL JOURNAL OF TUBERCULOSIS AND LUNG DISEASE | 2012年 / 16卷 / 01期

关键词：

MDR-TB; XDR-TB; drug resistance; GenoTypee MTBDRsl; RESISTANT MYCOBACTERIUM-TUBERCULOSIS; QUALITY-ASSURANCE PROGRAM; CAPREOMYCIN RESISTANCE; ETHAMBUTOL RESISTANCE; LABORATORY NETWORK; MUTATIONS; KANAMYCIN; RIFAMPIN; STRAINS; TLYA;

D O I：

10.5588/ijtld.10.0600

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

BACKGROUND: The GenoType (R) MTBDRsl assay is a new rapid assay for the detection of resistance to second-line anti-tuberculosis drugs. OBJECTIVE: To evaluate the MTBDRsl assay on 342 multidrug-resistant tuberculosis isolates for resistance to ofloxacin (OFX), kanamycin (KM), capreomycin (CPM) and ethambutol (EMB), to compare the results to the agar proportion method, and to test discrepant results using DNA sequencing. RESULT: The sensitivity and specificity of the MTBDRsl assay were respectively 70.3% and 97.7% for OFX, 25.0% and 98.7% for KM, 21.2% and 98.7% for CPM and 56.3% and 56.0% for EMB. DNA sequencing identified mutations that were not detected by the MTBDRsl assay. The 8/11 phenotypically OFX-resistant isolates had mutations in gyrA (2/8 had an additional mutation in the gyrB gene), 1/11 had mutations only in the gyrB gene, 6/21 phenotypically KM-resistant isolates had mutations in the rrs gene, and 7/26 and 20/26 phenotypically CPM-resistant isolates had mutations in the rrs and tlyA genes. CONCLUSION: The MTBDRsl assay showed lower sensitivity than previous studies. The assay performed favourably for OFX; however, it was less sensitive in the detection of KM/CPM resistance and demonstrated low sensitivity and specificity for EMB resistance. It is recommended that the MTBDRsl assay include additional genes to achieve better sensitivity for all the drugs tested. KEY WORDS: MDR-TB; XDR-TB; drug resistance; GenoType (R) MTBDRsl

引用

页码：104 / 109

页数：6